Protein Info for QEN71_RS02730 in Paraburkholderia sabiae LMG 24235

Annotation: EamA family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 19 to 21 (3 residues), see Phobius details amino acids 33 to 51 (19 residues), see Phobius details amino acids 61 to 82 (22 residues), see Phobius details amino acids 88 to 108 (21 residues), see Phobius details amino acids 116 to 134 (19 residues), see Phobius details amino acids 140 to 159 (20 residues), see Phobius details amino acids 168 to 190 (23 residues), see Phobius details amino acids 202 to 226 (25 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 264 to 286 (23 residues), see Phobius details PF00892: EamA" amino acids 6 to 131 (126 residues), 56 bits, see alignment E=2.5e-19 amino acids 142 to 280 (139 residues), 61 bits, see alignment E=7.1e-21

Best Hits

Swiss-Prot: 56% identical to EAMA_SALTY: Probable amino-acid metabolite efflux pump (eamA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03298, drug/metabolite transporter, DME family (inferred from 93% identity to bph:Bphy_0518)

MetaCyc: 54% identical to cysteine/O-acetylserine exporter EamA (Escherichia coli K-12 substr. MG1655)
RXN0-1923; RXN0-1924

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>QEN71_RS02730 EamA family transporter (Paraburkholderia sabiae LMG 24235)
MSPKDLLLALVVVVAWGVNFVVIKVGLHGVPPMLLGALRFMLAAFPAVFFVRRPQMPWRW
LLAYGATISLGQFAFLFSAMYVGMPAGLASLVLQAQAFFTLGFAALFLHERFRAQNVLGL
VIAAIGLAVIGLQGGHAMTVAGFVLTLCAAVMWALGNIVTKKVGKVDLVGLVVWGSLIPP
VPFFVLSYMFEGPARIEAALTGIGASSVFAIVYLAFVATLLGYGLWSRLLSRYPAGQVAP
FSLLVPIVGLASASVFLGEALSGAQILGAVLVMAGLAVNVFGGWVLQRFSLAR