Protein Info for QEN71_RS02230 in Paraburkholderia sabiae LMG 24235

Annotation: glycerol kinase GlpK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR01311: glycerol kinase" amino acids 4 to 495 (492 residues), 750.5 bits, see alignment E=3.2e-230 PF00370: FGGY_N" amino acids 5 to 251 (247 residues), 287 bits, see alignment E=1.3e-89 PF02782: FGGY_C" amino acids 261 to 449 (189 residues), 148.6 bits, see alignment E=2.1e-47

Best Hits

Swiss-Prot: 97% identical to GLPK_PARP8: Glycerol kinase (glpK) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 97% identity to bph:Bphy_0419)

MetaCyc: 60% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (499 amino acids)

>QEN71_RS02230 glycerol kinase GlpK (Paraburkholderia sabiae LMG 24235)
MQDQYILALDQGTTSSRAMLFDRKGNVVSVAQKEFRQIYPHPGWVEHDPQEIWATQAGVA
AEAVTHAGLNGTSIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGL
EDKVRAKTGLPIDSYFSATKIRWILDNVDGAREKAKQGKLAFGTVDSWLVWNFTKHELHI
TDVTNASRTMLFNIHTLQWDDELLDALDIPRSMLPEVRPSSEVYGPTKTTVFASKIPLAG
IAGDQHAALFGQMCTRSGMVKNTYGTGCFLVMNTGTKPIESKNNLVTTIAWQIGDQVNYA
LEGSIFIAGAVVQWLRDGLGIIRSASEVETLARGVEHCDGVYLVPAFAGLGAPHWNARAR
GTLFGVTRGTTSAHIARAALDSIAYQSVDVLKAMEADSGIHIDELRVDGGACANNLLMQF
QADILGVDAVRPKVSETTALGAAYLAGLATGYWKDIDELQKQWKLDHRFSPSMPAADAKA
CLDGWQRAIRAAKAWADAP