Protein Info for QEN71_RS01755 in Paraburkholderia sabiae LMG 24235

Annotation: RNA polymerase factor sigma-54

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 PF00309: Sigma54_AID" amino acids 4 to 48 (45 residues), 53.1 bits, see alignment 3.4e-18 TIGR02395: RNA polymerase sigma-54 factor" amino acids 9 to 503 (495 residues), 470.7 bits, see alignment E=2.6e-145 PF04963: Sigma54_CBD" amino acids 141 to 332 (192 residues), 200.6 bits, see alignment E=3e-63 PF04552: Sigma54_DBD" amino acids 348 to 503 (156 residues), 220.5 bits, see alignment E=1.6e-69

Best Hits

Swiss-Prot: 66% identical to RP54_CUPNH: RNA polymerase sigma-54 factor (rpoN) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K03092, RNA polymerase sigma-54 factor (inferred from 98% identity to bph:Bphy_0326)

Predicted SEED Role

"RNA polymerase sigma-54 factor RpoN" in subsystem Flagellar motility or Flagellum or Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (505 amino acids)

>QEN71_RS01755 RNA polymerase factor sigma-54 (Paraburkholderia sabiae LMG 24235)
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVSMAIAQNPLLEAEDDWIASPLRV
AADGTVIAQTAQNAAPEEMSSAPPSSSSTSSSESSDGGEPQGVDEYNGMSSDANGDSSQW
NLDDYGRSGNASDDDDLPPLQIHESTTTLRDHLTAQLRVTQASPRDRALVTFLIESLDDD
GYLTATFDEILADLPEELEVDTDELNAALALLHSFDPAGVGARSASECLRLQLCRLDGSP
TRTLALDIVAHHLELLAARDFTRLRKYLKASDDDLRDAHALIRSLEPFPGAAYGKAEADY
VVPDIMVRKTAQGWQAELNPEVVPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIK
NIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLGLHESTVSRVTTGKYML
TPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDTKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL