Protein Info for QEN71_RS01135 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 602 transmembrane" amino acids 324 to 347 (24 residues), see Phobius details PF20423: AceK_regulatory" amino acids 17 to 321 (305 residues), 392 bits, see alignment E=1.8e-121 PF06315: AceK_kinase" amino acids 322 to 576 (255 residues), 400.5 bits, see alignment E=3.2e-124

Best Hits

Swiss-Prot: 97% identical to ACEK_PARP8: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 97% identity to bph:Bphy_0180)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (602 amino acids)

>QEN71_RS01135 bifunctional isocitrate dehydrogenase kinase/phosphatase (Paraburkholderia sabiae LMG 24235)
MNHFPKLLSSQIGFDVAETLLAGFDRHYCIFREAAIRAKDLFEAADWHGLQKLARDRITS
YDERVRECIETLEDEYDAENIDDGVWQQIKLHYIGLLTTHRQPECAETFFNSVCCHILHR
SYFNNDFIFVRPAISTEYIENDEPAAKPTYRAYYPGKDGLAVTLERIVTNFQLNPPFENL
TRDVQCVIQALRDNFGTFNEAPNFQIHVLSSLFFRNKAAYIIGRIINGDMMVPFALPIHH
VKPGLLALDALLCKRDQLLIIFSFAHSYFLVDMEVPSAYVEFLGGILQGKPKAEIYTSVG
LQKQGKNLFYRDLLRHLKHSSDRFIIAPGIKGMVMLVFTLPSFPYVFKLIKDAFPPPKET
TREQVVGKYQLVKRHDRLGRMADTLEYSSVALPLSRLDDALIRELEKEAPSMLEYEGDNL
VIRHVYIERRMVPLNLFLQNGTDADIDHGIREYGNAVKELMQANIFPGDMLYKNFGVTRH
GRVVFYDYDEIEYLTDCNVRAVPPPRNEEDEMSGEPWYSVGPHDIFPETYHTFLLGDPRV
RESFLKHHADFFDPALWQKHKDHILRGELPDFYPYDRSLRFSVRYPERFAADRTAAPAER
AA