Protein Info for Psyr_5117 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: glutamine--fructose-6-phosphate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 611 (610 residues), 862.1 bits, E=1.1e-263
• PF13522: GATase_6
  • amino acids 67 to 185 (119 residues), 70.9 bits, E=1.6e-23
• PF13537: GATase_7
  • amino acids 83 to 194 (112 residues), 55.5 bits, E=8.3e-19
• PF01380: SIS
  • amino acids 291 to 419 (129 residues), 109.9 bits, E=1.3e-35
  • amino acids 464 to 593 (130 residues), 89 bits, E=3.7e-29

Best Hits

Swiss-Prot: 97% identical to GLMS_PSESM: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 100% identity to psb:Psyr_5117)

MetaCyc: 63% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See Q4ZL28 at UniProt or InterPro

Protein Sequence (611 amino acids)

>Psyr_5117 glutamine--fructose-6-phosphate transaminase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MCGIVGAVAERNITAILLEGLKRLEYRGYDSAGVAVFSNEGSLERRRRSGKVSELEQALA
GEPLIGRLGIAHTRWATHGAPCERNAHPHFSADTLAVVHNGIIENHEALREQLKGLGYVF
ASDTDTEVIVHLLHHKLKDTADLAVALKAAVKELHGAYGLAVINAAQPDRLLAARSGSPL
VVGMGLGENFLASDQLALRQVTDRFMYLEEGDIAEIRRDSVQIWNVDGVSVEREVVQYHE
GAEAADKGEYRHFMLKEIHEQPKVVQRTLEGRLGQQQVLVHAFGPQAAELFAKVRNVQIV
ACGTSYHAGMVARYWLEGLAGIPCQVEVASEFRYRKVVVQPDTLFVSISQSGETADTLAA
LRNAKELGFLASLAICNVGISSLVRESDLTLLTQAGPEIGVASTKAFTTQLVALLLLTLS
LGQVKGSLEEGVEAQLVEELRRLPTRLGEALAMDGTVEKVAELFAEKHHTLFLGRGAQFP
VAMEGALKLKEISYIHAEAYPAGELKHGPLALVDADMPVVTVAPNNELLEKLKSNLQEVR
ARGGELIVFADEQAGMKNGEGTHVIAMPHIIDALAPILYTIPLQLLSYYVAVLKGTDVDQ
PRNLAKSVTVE