Protein Info for Psyr_5092 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 289 to 309 (21 residues), see Phobius details PF16591: HBM" amino acids 33 to 287 (255 residues), 227.4 bits, see alignment E=3.1e-71 PF00672: HAMP" amino acids 309 to 360 (52 residues), 40.6 bits, see alignment 3.8e-14 PF00015: MCPsignal" amino acids 424 to 606 (183 residues), 153.7 bits, see alignment E=6.8e-49

Best Hits

Swiss-Prot: 64% identical to MCPS_PSEPK: Methyl-accepting chemotaxis protein McpS (mcpS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03406, methyl-accepting chemotaxis protein (inferred from 100% identity to psb:Psyr_5092)

Predicted SEED Role

"Methyl-accepting chemotaxis protein I (serine chemoreceptor protein)" in subsystem Bacterial Chemotaxis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZL53 at UniProt or InterPro

Protein Sequence (640 amino acids)

>Psyr_5092 Histidine kinase, HAMP region:Bacterial chemotaxis sensory transducer (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNSRFANISVNMKLALGFGTVLFFTAVLALVGWTSLDKLIYRTDRIGDITRLSDNLTNLR
VARLQYMLTDGDETAAQNMQSKLDVFKAQQQSLLVSFNNPVNLKPLRELADVTRDYEASL
NSMRAVYQAGAKVRNEMTANGTAAMQAVESLNNAVLQIDPADPARFDLAQLANSARQDLV
LVRYEVRGYTGNPNDKTETAAFQQLDSAISHLDRFKAAFGPANREQIAQFESALRNYRSS
VDVFKATTQTAASVRKDLTTQGATIVKLGEELYGLQMQMGKEDTAHARSLQIGCVVLVML
LGILAAVIITRQITRPLRDTLAIVERIASGDLTHTEAVTRRDELGVLQQGIQRMGTTLRE
LISGIRDGVTQIASAAEELSAVTEQTSAGVNSQKVETDQVATAMHEMSATVHEVARNAEQ
ASVAASDADQQARDGDKVVGEAIQQIERLAAEVVRSSDAMNVLEQDSDKIGKVMDVIKAV
AEQTNLLALNAAIEAARAGEAGRGFAVVADEVRGLAQRTQQSTEEIEGLVAALQNGTRQV
SGIMLGSRTLTDSSVELTRRAGTSLESITRTVSSIQAMNQQIAAAAEQQSSVADEISRSI
VNVRDVSEQTAEASEETAASSVELARLGGQLQMMVSHFRV