Protein Info for Psyr_4824 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: ABC transporter, substrate-binding protein, aliphatic sulfonate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13379: NMT1_2" amino acids 22 to 243 (222 residues), 35.8 bits, see alignment E=2.5e-12 TIGR01728: ABC transporter, substrate-binding protein, aliphatic sulfonates family" amino acids 28 to 310 (283 residues), 279.6 bits, see alignment E=1.3e-87 PF04069: OpuAC" amino acids 52 to 234 (183 residues), 23.4 bits, see alignment E=1.2e-08 PF12974: Phosphonate-bd" amino acids 66 to 229 (164 residues), 30 bits, see alignment E=1.1e-10 PF09084: NMT1" amino acids 67 to 230 (164 residues), 49.4 bits, see alignment E=1.7e-16 PF16868: NMT1_3" amino acids 106 to 181 (76 residues), 30.9 bits, see alignment E=6.3e-11 PF00497: SBP_bac_3" amino acids 110 to 230 (121 residues), 28.2 bits, see alignment E=4.1e-10

Best Hits

Swiss-Prot: 62% identical to SSUA_ECOLI: Putative aliphatic sulfonates-binding protein (ssuA) from Escherichia coli (strain K12)

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to psb:Psyr_4824)

MetaCyc: 62% identical to aliphatic sulfonate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-56-RXN [EC: 7.6.2.14]

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZLX1 at UniProt or InterPro

Protein Sequence (311 amino acids)

>Psyr_4824 ABC transporter, substrate-binding protein, aliphatic sulfonate (Pseudomonas syringae pv. syringae B728a ΔmexB)
MKPFTFIAAILAFSGLFGTASADEPDTLRIGYQKGSITLVLAKENGLLEKRFPEKKIKWV
EFPAGPQMLEALNIGSLDIASTGDIPPIFAQVAGADLVYIGAEPPKPQVETLLVRNESPV
HSVAELKGRKVALQKGSSSHNVVLRVLNKAGLTFKDIQPIYLTPADARAAFENGSVDAWA
IWDPYYSIAMSEGHSRLLANGEGLGLSGPFYTARRAFAEENAALVQQVLDELTVADGLSR
SQRAESIQILARSMGLSEAVMTQYLDHRPVSPSLPITPEIVRAQQATADLFYDNHLIPKR
VDIQQVVWQKP