Protein Info for Psyr_4792 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: transketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00456: Transketolase_N
  • amino acids 5 to 333 (329 residues), 535.9 bits, E=8.5e-165
• TIGR00232: transketolase
  • amino acids 6 to 663 (658 residues), 1006.7 bits, E=2.3e-307
• PF00676: E1_dh
  • amino acids 111 to 243 (133 residues), 23.8 bits, E=5.3e-09
• PF02779: Transket_pyr
  • amino acids 353 to 524 (172 residues), 189.8 bits, E=8.4e-60
• PF02780: Transketolase_C
  • amino acids 540 to 655 (116 residues), 59.8 bits, E=6.6e-20

Best Hits

Swiss-Prot: 72% identical to TKT1_ECOLI: Transketolase 1 (tktA) from Escherichia coli (strain K12)

KEGG orthology group: K00615, transketolase [EC: 2.2.1.1] (inferred from 100% identity to psb:Psyr_4792)

MetaCyc: 72% identical to transketolase 1 (Escherichia coli K-12 substr. MG1655)
Transketolase. [EC: 2.2.1.1]; 2.2.1.1 [EC: 2.2.1.1]

Predicted SEED Role

"Transketolase (EC 2.2.1.1)" in subsystem Calvin-Benson cycle or Pentose phosphate pathway (EC 2.2.1.1)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (15/17 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (non-oxidative branch) I (5/5 steps found)
• Bifidobacterium shunt (12/15 steps found)
• pentose phosphate pathway (partial) (3/3 steps found)
• Rubisco shunt (8/10 steps found)
• pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
• Calvin-Benson-Bassham cycle (10/13 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (6/9 steps found)
• oxygenic photosynthesis (11/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Carbon fixation in photosynthetic organisms
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.1

Sequence Analysis Tools

See Q4ZM03 at UniProt or InterPro

Protein Sequence (665 amino acids)

>Psyr_4792 transketolase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MPSRRERANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWRDYLKHNPANPSFADRDRF
IMSNGHGSMLVYSLLHLTGYDLSIDDLKNFRQLHSRTPGHPEYGYTPGIETTTGPLGQGF
ANAVGFAAAEKTLAAQFNRPGHSIVDHHTYVFMGDGCMMEGISHEAASLAGTLQLNKLIA
FYDDNGISIDGEVEGWFTDDTPKRFESYGWLVIRNVDGHDPEEIKTAIETARKSDQPTLI
CCKTTIGFGSPNKQGKEESHGAPLGADEIALTRAALKWNYGPFEIPADIYAEWNGKEKGL
AAEAEWDQRFADYSAAFPELANDFIRRMSGQLPADFAEKSAAYVAEVNAKGETIASRKAS
QNALGALGPLLPEILGGSADLAGSNLTIWKGCKSITGEDPNGNYLHYGVREFGMGAMMNG
IALHGGFVPYGATFLIFMEYARNAVRMASLMKKRVIYVFTHDSIGLGEDGPTHQPIEQLT
SLRTTPNLDTWRPADAVESAVAWKHAIERDDGPSALIFSRQNLTHQARDEKQLSYIARGG
YVLRDCAAEPELILIATGSEVGLAVQAYDKLTEQGRNVRVVSMPCTSIFEAQDAAYKQAV
LPLQVSARIAIEAAHADYWYKYVGLEGRVIGMTTFGESAPAPALFEEFGFTLENVLSTAE
ELLED