Protein Info for Psyr_4755 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: hydrolase, alpha/beta fold family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 344 PF12146: Hydrolase_4" amino acids 65 to 286 (222 residues), 43 bits, see alignment E=5.3e-15 PF00561: Abhydrolase_1" amino acids 67 to 315 (249 residues), 83.7 bits, see alignment E=2.6e-27 PF12697: Abhydrolase_6" amino acids 69 to 313 (245 residues), 43 bits, see alignment E=1.3e-14

Best Hits

KEGG orthology group: K07019, (no description) (inferred from 100% identity to psb:Psyr_4755)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZM40 at UniProt or InterPro

Protein Sequence (344 amino acids)

>Psyr_4755 hydrolase, alpha/beta fold family (Pseudomonas syringae pv. syringae B728a ΔmexB)
MPTRPTFVSHANPFVPAVGMGNPHLQTLWGPLLRKPTLLARTRERLWLQDGDFLDMDWHG
PDQPDKPLVLVLHGLTGSSNSPYVAGLQKAMAALGWPSVALNWRGCSGEPNLLSRSYHSG
ASEDLAEVIAHLRSLRPLAPIYAVGYSLGGNVLLKYLGESGANSDLRGAVAVSVPFRLDE
CANRIGQGFSRVYQRHFMREMLAYIRDKQHRFQHEGISEGLAELAALGSLENMRTFWDFD
GRVTAPLHGFTDATDYYRRASSRYYLGQIQTPTLIIQSSDDPFVFPHSLPEPSELSSCTE
FELHTKGGHVGFVEGSLRTPRYYLERRIPLWLCAAQEREGSPTI