Protein Info for Psyr_4629 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Bis(5'-nucleosyl)-tetraphosphatase (symmetrical)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF00149: Metallophos" amino acids 1 to 82 (82 residues), 47.9 bits, see alignment E=1.1e-16 TIGR00668: bis(5'-nucleosyl)-tetraphosphatase (symmetrical)" amino acids 1 to 259 (259 residues), 306 bits, see alignment E=1.3e-95

Best Hits

Swiss-Prot: 100% identical to APAH_PSEU2: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01525, bis(5'-nucleosyl)-tetraphosphatase (symmetrical) [EC: 3.6.1.41] (inferred from 100% identity to psb:Psyr_4629)

MetaCyc: 50% identical to diadenosine tetraphosphatase (Escherichia coli K-12 substr. MG1655)
Bis(5'-nucleosyl)-tetraphosphatase (symmetrical). [EC: 3.6.1.41]; Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.41, 3.6.1.40]

Predicted SEED Role

"Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)" (EC 3.6.1.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40 or 3.6.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZMG3 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Psyr_4629 Bis(5'-nucleosyl)-tetraphosphatase (symmetrical) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MAVYAVGDLQGCLEPLQCLLEHVHFNPEQDRLWLVGDLVNRGPQSLETLRYLYSLRESLV
CVLGNHDLHLLAVARKKELLKKGDTLLEILEAPDRDDLLGWVRRQKLMHYDAQRNVAMVH
AGIAPQWTLKKALKHAAEVEHALQDEQLYGAFLDGMYGNEPTRWDNDLQGVTRLRVITNY
FTRMRFCTSDGKLDLKSKEGVGTAIPGYAPWFSHQSRKTRDVKIIFGHWAALEGRCDEPD
VFALDSGCVWGGSMTLLNVDTLERHQCNCDAMGNAADGLVTRVQPGATPLP