Protein Info for Psyr_4456 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00505: GTP cyclohydrolase II
  • amino acids 23 to 210 (188 residues), 263.4 bits, E=5e-83
• PF00925: GTP_cyclohydro2
  • amino acids 24 to 186 (163 residues), 226 bits, E=1e-71

Best Hits

Swiss-Prot: 98% identical to RIBA_PSESM: GTP cyclohydrolase-2 (ribA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K01497, GTP cyclohydrolase II [EC: 3.5.4.25] (inferred from 100% identity to psb:Psyr_4456)

MetaCyc: 66% identical to GTP cyclohydrolase 2 (Escherichia coli K-12 substr. MG1655)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25

Use Curated BLAST to search for 3.5.4.25

Sequence Analysis Tools

See Q4ZMY6 at UniProt or InterPro

Protein Sequence (221 amino acids)

>Psyr_4456 GTP cyclohydrolase II (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQILRISSLHTDQEHTVPVVFVAASKLPTPFAEFAMHGFIESATGREHVVLSLGDVADGE
PVLGRVHSECLTGDALFSQRCDCGSQLEAALRAIATEGRGVLLYLRQEGRGIGLMNKIRA
YELQDGGADTVEANERLGFAADQRDYAICLPMLQHLGVQSLRLMTNNPRKVKALTEMGIR
VAERVPLHTGQNPHNRLYLATKAGKLGHMMGNEHQSEVGPA