Protein Info for Psyr_4312 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Erythronolide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08659: KR
  • amino acids 237 to 416 (180 residues), 137 bits, E=3.9e-43
• PF00106: adh_short
  • amino acids 239 to 410 (172 residues), 45.8 bits, 2.8e-15
• PF13489: Methyltransf_23
  • amino acids 676 to 832 (157 residues), 32.2 bits, 4.6e-11
• PF13649: Methyltransf_25
  • amino acids 695 to 798 (104 residues), 31.9 bits, 1e-10
• PF08242: Methyltransf_12
  • amino acids 696 to 799 (104 residues), 52.7 bits, 3.2e-17
• PF08241: Methyltransf_11
  • amino acids 696 to 802 (107 residues), 34.8 bits, 1.2e-11
• PF00550: PP-binding
  • amino acids 924 to 985 (62 residues), 42.8 bits, see alignment (E = 2.9e-14)
  • amino acids 2177 to 2242 (66 residues), 33.1 bits, see alignment (E = 2.9e-11)
• PF00109: ketoacyl-synt
  • amino acids 1069 to 1312 (244 residues), 254 bits, E=9.5e-79
• PF02801: Ketoacyl-synt_C
  • amino acids 1320 to 1440 (121 residues), 123.1 bits, 3.4e-39
• PF16197: KAsynt_C_assoc
  • amino acids 1469 to 1543 (75 residues), 43 bits, see alignment (E = 3e-14)

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_4312)

Predicted SEED Role

"Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 2.3.1.39)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• fatty acid biosynthesis initiation (mitochondria) (2/4 steps found)
• fatty acid biosynthesis initiation (plant mitochondria) (1/4 steps found)
• mycobactin biosynthesis (3/11 steps found)
• pederin biosynthesis (2/14 steps found)
• bryostatin biosynthesis (2/19 steps found)
• mupirocin biosynthesis (2/26 steps found)
• corallopyronin A biosynthesis (2/30 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.39

Use Curated BLAST to search for 2.3.1.39

Sequence Analysis Tools

See Q4ZND0 at UniProt or InterPro

Protein Sequence (2260 amino acids)

>Psyr_4312 Erythronolide synthase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSNAVHNIYAVNRWRPIAGSPSLATDESVLVIIPVTSAAGVSPNRQRLLSWVGGQAHCSF
TALDTLERMTATLADCIPGKIKSILWIGGSLDVSVHAEDQGLQYGIEEKIFFRFFKALLE
LKADEVRLSITVVTKNNFNVNNEGTNTAVNAGLHGLVGAAAKEYSHWTISLLDVDDAFVA
RATSNPSLVRSSQGIEKRKVKGQTYCCRDDQWYAEHLEVLPVENGSGPVKPIYRDNGVYL
IIGGAGGLGQAWTESLLQRHDSQVIWAGRKAINDEIRHSLRAMERLGRAPLYVSVDATQP
HQMEQLRHTILRQYGRLDGIIHSAIVLEDKTVANMSETTFSSALAAKVDICRNIENYFAD
LDLDFIAFYSSIQSFARQAGQSNYAAGCTFKDSFAHYLQAKCATTHVRTMNWGYWGTVGI
VSDASYQKVLEKSGMASINPTIGMLAFKALMAGDHLQASFIAAYDPEKITLIDKVTLHQN
TTEFGFVQSTTQLNNNVVEGCMMEYVYRNEHYMALREEGVFYLEDMDSYLCRIVAADLET
LGVFSGTEFSVENVLRKTDIRAGFSRWLTETLMSLVRQGYLRHDAGQFSKTAEFPESDAS
AAWLSWNTAKVEWLLDDNKSAQVKLVEALLIHTKEILTGKIKATDIMFPDSSMRMVEGIY
KNNRVADYFNLVLSRTVVDEINTLLAADPQRKIRILEVGAGTGGTTAVLLKHLAPFHANI
AEYCYTDLSKSFLFHAQREYGPGNPFLRYEIFNVEKSVDDQPLQRDYYDIAVATNVLHAT
SDIAASIRRVWEVLKDQGSLIVNELSRNTLFNHLTFGFLDGWWLYNDDHIRVPGSPVLEP
ATWHNTALQVGYGSTQFPAELAHDMGQFVMLMRAQKHAASLAPKVVESGSSHSSGNESSM
LTPSNTPSAVNVAMLKAKSQAFFTDLIADVVQLPVDEIALDLSLEKLGFDSILVVTLTNK
LREHFEQVSSSLLFDIDSVEGIIDYFIEQDKQALAALLGIDFSDAQPPSAAPVAVASHTQ
APASAPAVTQFDVIDLGAFSLQEVDAPSASLPQVSDFQETYPEPRRSGDIAIIGLNGTYP
GSRTLDEFWENLSQGRDLVTSVPKARWEQTGFNRVENPENFKGGFIDGFDQFDAEFFDFS
ETDALLTDPQERKFLECAFSVLEDAGYTKESLAESDMTTGVYVGAMYQEYQLHSETAKGL
YEGQAISNSFASIANRVSYILNLEGPSVAIDSMCSSSLSAVHLACSALKLGEIDYAIAGG
VNLTLHPNKYGVLKQGGDLSQTGKCASFSESADGYVPGEGVGAVFLKRYEDALRDGDHIY
GVITGSLIVHAGKTNGYTVPSPKAHSKLISKVVQQAGIRSADISYIEAHGTGTHLGDAIE
IHGLRQAFSIDQDSAQQGKFCSVGSVKSNIGHCESAAGMAGLTKILLQLKYQQIVPSLHS
KKINKNLQIEKTSFSIPQLLSHWRLPNKEVKKRIAGLSSFGAGGANAHFIIEEHEEVSNQ
VNYTMDYPELIVLSAKSKEALHQRAAQLLGYIHYNAQQNTSLNNQPRYWLGNIAYTLQTG
RTQMPYRLSFLADSIEHLEDNLDAFLNDKKGKSPLHVCYASEMDATAKLLYEDNSLELIL
SEWLKQGKYQNIVALWAKGVVINWGGLLGKRGQRISLPTYPFSQNRHWLPMGNHTAGVSQ
KVEAASVQQRVKTVAAMVEPVVEKVAGKIVQKTVEPVVGKPFITAPVAVEKVTAEPKSLF
IHRWNKVDVSPHPKPAQADTKICVVGLDTLDYFQRDLETCGLDAGSSEWQQVLPNGFAQS
FADLAKSVSRQLAKKVKAWLADSTQPLHLQIIMPFDRQYQLYAALTGALEQHVLGAANVK
LTIIYCEALCSFANISTLINRAADTQTHIAEYLCAHDSIYAGSVQKVSQPESGSLLNDNC
VYVLANGLNEVGQSVIRAIDDETSGCHIAILGDALSAPDIDEQIGLLQNSLDNTKVGYYA
AELDDRKSVFNALLLIKDQLGDIQGVHYIVPADDKVQQHKEPLKDKALIRHLAALINLDE
ATYSFDSQLQSFSIVMTVEEHDSKQLNTLAKTFGRYRAALQGTGYREGKTVALEVEGNAA
NDIATAFIHALDKNHSDEQFAFIWKAATLADNTPVIQPQVTVEPPVASPAPLAAVAQKQP
AYSFDGASDELQATVSWLVSISSELLDKESASFSPVATFTENGYESISIVGLCELINSRL
GLAISVVVFFEHDTIQSLAQYLVAEHGDQIEEVLETDVEG