Protein Info for Psyr_4297 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Helicase, C-terminal:DEAD/DEAH box helicase, N-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF00270: DEAD" amino acids 25 to 194 (170 residues), 162 bits, see alignment E=1.7e-51 PF04851: ResIII" amino acids 25 to 190 (166 residues), 29.6 bits, see alignment E=9.6e-11 PF00271: Helicase_C" amino acids 231 to 339 (109 residues), 102 bits, see alignment E=3.4e-33

Best Hits

Swiss-Prot: 57% identical to RHLE_ECOLI: ATP-dependent RNA helicase RhlE (rhlE) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_4297)

MetaCyc: 57% identical to ATP-dependent RNA helicase RhlE (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase PA3950" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNE5 at UniProt or InterPro

Protein Sequence (442 amino acids)

>Psyr_4297 Helicase, C-terminal:DEAD/DEAH box helicase, N-terminal (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTFASLGLIEPLLRALEALGYQTPTPVQTQAIPPVLAGRDLMAAAQTGTGKTAGFALPLL
QRLTMEGPKVAPNSIRALVLAPTRELADQVHESIRQYAEHLPLTTYAAYGGVSINPQMMK
LRRGVDVLVATPGRLLDLHRQNAVKFSQLQTLILDEADRMLDLGFAEELRGIYAVLPKQR
QTLLFSATFSDEIRLLAAQMLNDPLTIEVSPRNVAASSVKQWVVTVDKKRKADLFIHLMK
KHRWGQVLVFAKTRVGVDQLVDRLQALGMNADGIHGDKPQATRQRALDRFKSNEVKILVA
TDVAARGLDIDDLPTVVNLDLPIVAEDYIHRIGRTGRAGLTGEAISLVCADEVELLSAIE
VLTRQTLERKEEQDFEPEHRVPSTDASGQILKKPKKPKKSKVSGSKRNLGKWVDSGDSEP
APVVKPVRKVPVFNTGPRKKKP