Protein Info for Psyr_4265 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1278 transmembrane" amino acids 27 to 45 (19 residues), see Phobius details amino acids 253 to 275 (23 residues), see Phobius details amino acids 295 to 311 (17 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 446 to 575 (130 residues), 32 bits, see alignment E=1.2e-11 amino acids 578 to 717 (140 residues), 52.3 bits, see alignment E=6.2e-18 amino acids 722 to 838 (117 residues), 56.7 bits, see alignment E=2.6e-19 PF00989: PAS" amino acids 583 to 707 (125 residues), 33.1 bits, see alignment E=1.8e-11 amino acids 722 to 829 (108 residues), 41.4 bits, see alignment E=4.4e-14 PF13426: PAS_9" amino acids 595 to 709 (115 residues), 33.5 bits, see alignment E=1.5e-11 amino acids 729 to 831 (103 residues), 64.4 bits, see alignment E=3.7e-21 PF08448: PAS_4" amino acids 724 to 833 (110 residues), 34.6 bits, see alignment E=6.7e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 840 to 1006 (167 residues), 140.4 bits, see alignment E=4.6e-45 PF00990: GGDEF" amino acids 843 to 1004 (162 residues), 168.7 bits, see alignment E=3.3e-53 PF00563: EAL" amino acids 1024 to 1259 (236 residues), 263.3 bits, see alignment E=6.3e-82

Best Hits

KEGG orthology group: None (inferred from 77% identity to pfl:PFL_5345)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNH7 at UniProt or InterPro

Protein Sequence (1278 amino acids)

>Psyr_4265 diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MPTSTPLSSSSVATDSATRPLRGSVKGMLAGLVLVVLALLLWQLIEQFHQTQERQRQRSL
EYSVQLSDRMSLNMALKAQIAMNLLDETLSSGEQLEQPTTLLNLRTALPALRSVARLNPA
GEIVSDSAEASRDAEFLGRAMKQARGRPYFYTNSEDGNQIYILIRLGNGVSGDYWALRVA
PDALKELASQPAQDFQYLWRLEQRKTQRVILHEPVAANDADSGLSDTILLQPLSNSDWQL
RGLFDTYKARSELLAPLIGKCLIGVLLSILPLLALMSLRRRQRQMLQSRRRYRDIFEGAG
VAICVMDMSGLQEQLEALEVRNGEDFERLLKSQPASLKRLLQQLRISELNEVALSLLDIE
HREQAWQPLIEGESRSRPGVGIPIIQAVLAGQPRLELEICLTRAGGQDQHLWLVMRLPET
RGDFDAVILSIGDITSRKQVELSLLDRESFWSDVVQTVPDHLYVQDVLSQRVIYSNHHFG
YTLGYSKSELHAMGEFFWEVLLHPDDARHYQDMRLRQRHHGHNELLQSVLRFRDRESNWR
SFDIREQALAWDAEDRVTRIIGIAKDITEQVASSQSLRDSEQRYRMLAESISDVIFSTDG
RLAVNYVSPSVERALGYSAEWIIQNGWSAAIANPQQIVGFDALMSRIDKVLDKPQELARL
REEVTTRMFLFDCLRADGRKIPVELRVVLVWGEHGNFEGIFGVGRDISQQRRAEKDLRMA
ATVFEHSTSAILITDPAGYIVQANEAFTRVSGYEVAQVLDQLPGMLTVESEQETRLRHIL
RQLNGQGTWEGEIRLKRRNGEHYPAWVGITAVLDDEGDLASYVCFFSDISERKASEDRIH
RLAYYDALTHLPNRTLFQDRLHSALQHAERQQAWVVLMFLDLDRFKPINDSLGHAAGDRM
LQEMATRLLACVSVDDTVARMGGDEFTLLLEPDESREAALNRAIHVAERILTSLITPFVL
EGREFFVTASIGIALSPQDGKELSQLMKNADTAMYHAKERGKNNFQFYQADMNATALERL
ELESDLRHALEQKEFTLFYQPQFSGDGKRLTGAEALLRWRHPRRGLVPPNDFIPVLEELG
LVVEVGDWVLAEACRQLKHWHQARVRVPKVSVNISARQFSDGQLGKRIAHILEQAGLSPA
CLELELTESILMREVNEAMQILDGLKNLGLSIAVDDFGTGYSSLNYLKQFPIDVLKIDRT
FVDGLPSGEQDAQIARAIIAMAHSLNLSVIAEGVETHEQLEFLREHDCDEVQGYLFGRPM
PPAQFEAQFSNDALFMLD