Protein Info for Psyr_4154 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: microcin-processing peptidase 1, Unknown type peptidase, MEROPS family U62

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 245 to 265 (21 residues), see Phobius details PF01523: PmbA_TldD_1st" amino acids 34 to 98 (65 residues), 51.4 bits, see alignment E=1.5e-17 PF19290: PmbA_TldD_2nd" amino acids 126 to 232 (107 residues), 73.8 bits, see alignment E=2.5e-24 PF19289: PmbA_TldD_3rd" amino acids 239 to 447 (209 residues), 227 bits, see alignment E=2.6e-71

Best Hits

Swiss-Prot: 48% identical to PMBA_HAEIN: Metalloprotease PmbA homolog (pmbA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03592, PmbA protein (inferred from 100% identity to psb:Psyr_4154)

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZNT8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>Psyr_4154 microcin-processing peptidase 1, Unknown type peptidase, MEROPS family U62 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSATQSVGPQALPELQEQVEQIIAEARRQGASACEVAVSVDQGLSTSVRQREVETVEFNR
DQGFGITLYVGQRKGSASTSATGADAIRETVAAALAIAEHTSEDESSGLADAALMAKEVM
DFDLYHTWDITPEQAIEKALLCEAAAFDADSRIKNADGTTLNTHQGCRVYGNSNGFIGGY
ASTRHSLSCVMIAEGEGQMQRDYWYDVNRQGELLADAQSIGRKAALRAASRLGARPVPTC
EVPVLFAAELAGGLFGSFLGAISGGTLYRKSSFLEGALGQRLFPEWMTIDERPHLRHAMG
SSAFDSDGLATFAKPFVAGGDLVSYVLSTYSGRKLGMPSTANAGGVHNLFVSHGSDDQAA
LIRRMGRGLLVTELMGSGLNMVTGDYSRGAAGFWVENGEIQFPVQEVTIAGNMRDMFKQI
VAVGSDLELRSNIRTGSVLIERMMVAGS