Protein Info for Psyr_3996 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: PAS/PAC sensor hybrid histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 PF08448: PAS_4" amino acids 50 to 140 (91 residues), 25.6 bits, see alignment E=4.2e-09 amino acids 160 to 269 (110 residues), 51.6 bits, see alignment E=3.7e-17 PF13426: PAS_9" amino acids 165 to 267 (103 residues), 20.1 bits, see alignment E=2.3e-07 amino acids 304 to 396 (93 residues), 15.5 bits, see alignment E=6e-06 PF00989: PAS" amino acids 292 to 383 (92 residues), 28.3 bits, see alignment E=5.5e-10 TIGR00229: PAS domain S-box protein" amino acids 293 to 406 (114 residues), 45 bits, see alignment E=5.6e-16 PF08447: PAS_3" amino acids 315 to 384 (70 residues), 40.3 bits, see alignment E=1.1e-13 PF00512: HisKA" amino acids 423 to 486 (64 residues), 36.5 bits, see alignment E=1.4e-12 PF02518: HATPase_c" amino acids 529 to 653 (125 residues), 66.8 bits, see alignment E=8.2e-22 PF00072: Response_reg" amino acids 675 to 786 (112 residues), 70.6 bits, see alignment E=4.4e-23

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_3996)

Predicted SEED Role

"Sensory box histidine kinase/response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZP96 at UniProt or InterPro

Protein Sequence (796 amino acids)

>Psyr_3996 PAS/PAC sensor hybrid histidine kinase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MGRFIRETDWSTSALGPKQAWPAALLSSMNLMLSCPDSMYLLWGPDYTLFFNDAYAQVLP
IAAAQAQGQPIASVWGDAWEAVRPLAEQALAGQNRRSDNIQRTIVRNGKPEQTWWSLSYS
PLYGDSGLIEGVFCRVNETTLQVMAEARQSENEAFTDRVLSSINDCIKVLDLDSRLTFMS
EGGQRIMEVSDFNAIRGCPWPDFWQEQGNLDAIAAVEAARAGQNASFTGSARTLAGTTKW
WHVQVSPIFGKDGKPEKILCVSRDMTQLRDAEEALLSLNETLEQRVVERTQDRDRIWRLS
TDLMLVAQFDGIISAVNPAWTQALGWSEEQLLDSQFLALVHPEDVDSTLAAMSGLENGRT
IPHFKNRYRHQDGSYRTISWTAVPDKQFIHAVGRDIQAEEEANEALRLSQDALHQAQKLE
AIGQLTGGVAHDFNNLLTVIRSCSDLLKSPNLEEHRRVKYVEAISSTVDRAARLTGQLLA
FARRQALQPEVFDVCKSVARIGEMMDTLTGARIQVSIDLPAEPCFIFADGSQFDTALVNM
VVNARDAMSGAGRLVIKVECAACPADDQPASLGEGDYIEGDYVRVSLTDTGAGIAKEKLG
LIFEPFYTTKSVGQGTGLGLSQVFGFAKQSGGEVLVESELGKGSRFTLCLPSAQNHEVVQ
EDHRHTLTPLPGLCVLMVEDNQDIGTYTRPMLEQLGFQVLWVSSAAEALKELSGNPENFH
VVFSDIAMPGMSGLELYAEIEARYPWMPVVLTTGYSTEFATIAQDETQRFDLLQKPYSRD
DLAAILHKAVSRSGEH