Protein Info for Psyr_3930 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: quinoprotein glucose dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 807 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 42 to 59 (18 residues), see Phobius details amino acids 66 to 82 (17 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 16 to 806 (791 residues), 1208.6 bits, see alignment E=0 PF01011: PQQ" amino acids 229 to 257 (29 residues), 22.4 bits, see alignment (E = 1.2e-08) amino acids 312 to 344 (33 residues), 25.8 bits, see alignment (E = 9.6e-10) amino acids 739 to 771 (33 residues), 23.1 bits, see alignment (E = 7e-09) PF13360: PQQ_2" amino acids 238 to 537 (300 residues), 42 bits, see alignment E=1.4e-14 amino acids 687 to 774 (88 residues), 22.2 bits, see alignment E=1.5e-08

Best Hits

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 100% identity to psb:Psyr_3930)

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.2

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPG2 at UniProt or InterPro

Protein Sequence (807 amino acids)

>Psyr_3930 quinoprotein glucose dehydrogenase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSTDSASNRNRLLPTLLGLVILLMGLALLVGGARLLQLDGSLYYLLAGIGFAVTGVLLIT
GRAAALGLYALLLFASTVWSLWEVGLDWWQLVPRLALWFALGIVLLLPWFREPLLRNGPA
RMGTGALSIAVVLAGLTALASQFTNPGRIEGQLDRETAGTTNTAPAMPDGDWQSYGRTAF
GDRYSPLAQITPENVNKLEPAWTYRTGDIPGPNDPGETTAENTPLKVNGMLYVCTPHSQV
IALDPDSGKEIWRFDPKLSTQNAANFKGWAHMTCRGVTYHDDAAYAASAPAQSPTVPAAD
GTATASAACPRRIFLPTADTRLIALNADTGKMCEDFGNKGSVDLTANMGTFAPGGYYSTS
PPAVTKDLVIIGGHVTDNVSMDEPSGVIRAYDVHTGRLVWNWDSGNPEETAPIAEGKIYT
RNSPNMWSMFSVDEKLGMIYLPMGNQTPDQWGGDRTPESEKYSAGLVALDIATGRVRWDY
QFTHHDLWDMDVGGQPTLLDMKTADGIKPAVLASTKQGSIYVLDRSTGKPIVPINEVPVP
QGAVAGDHTSPTQPKSDLNFMPPPLKERDMWGVTPFDQMMCRIDFKSLRYDGPFTPPSLQ
GSIVYPGNFGVFDWGGISVDPVRQIAFVNPNYMAFRSKLVPSAEVEGGPGRKSETEGVQP
NKGAPYGVILEALLSPMGLPCQAPAWGYVAAVDLTTQKTIWMHKNGTVRDSSPIPLPLTM
GVPSLGGTFTTASGLAFMSATLDQYLRAYDVRNGKQLWEARLPAGAQTTPMTYTGKDGQQ
YVLVVAGGHGSLGTKQGDYVMAFKLPK