Protein Info for Psyr_3756 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Na+/solute symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 552 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 36 to 55 (20 residues), see Phobius details amino acids 77 to 99 (23 residues), see Phobius details amino acids 105 to 124 (20 residues), see Phobius details amino acids 150 to 170 (21 residues), see Phobius details amino acids 178 to 200 (23 residues), see Phobius details amino acids 211 to 229 (19 residues), see Phobius details amino acids 264 to 288 (25 residues), see Phobius details amino acids 300 to 325 (26 residues), see Phobius details amino acids 357 to 385 (29 residues), see Phobius details amino acids 407 to 426 (20 residues), see Phobius details amino acids 432 to 455 (24 residues), see Phobius details amino acids 466 to 489 (24 residues), see Phobius details amino acids 501 to 519 (19 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 65 to 470 (406 residues), 208.4 bits, see alignment E=9.8e-66 PF00474: SSF" amino acids 65 to 471 (407 residues), 436.9 bits, see alignment E=3.8e-135

Best Hits

Swiss-Prot: 83% identical to ACTP_YERE8: Cation/acetate symporter ActP (actP) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K14393, cation/acetate symporter (inferred from 100% identity to psb:Psyr_3756)

MetaCyc: 79% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZPY5 at UniProt or InterPro

Protein Sequence (552 amino acids)

>Psyr_3756 Na+/solute symporter (Pseudomonas syringae pv. syringae B728a ΔmexB)
MIRRLSAALGLAAFAPTLWAADALTGAVQKQPLNVAAIVMFVVFVAFTLYITYWASKKNK
TASDYYSAGGKITGFQNGLAIAGDYMSAASFLGISALVYTSGYDGLIYSIGFLVGWPIIL
FLIAERLRNLGKYTFADVASYRLKQKEIRTLSACGSLVVVAFYLIAQMVGAGKLIQLLFG
LDYAVAVVLVGILMCLYVLFGGMLATTWVQIIKAVMLLSGASFMALMVMKHVNFDFNMLF
SEAIKVHPKGEAIMSPGGLVKDPISAFSLGLALMFGTAGLPHILMRFFTVSDAKEARKSV
LYATGFIGYFYILTFIIGFGAILLVSTNPAFKDAAGALLGGNNMAAVHLADAVGGSLFLG
FISAVAFATILAVVAGLTLAGASAVSHDLYASVIKAGKANEKDEIRISKITTIALAVVAI
FLGIAFESQNIAFMVGLAFSVAASCNFPILLLSMYWKNLTTRGAMIGGWMGLISAVVLMV
LGPTIWVQILHHEKAIFPYEYPALFSIAIAFVGIWFFSITDKSKAAEGERALFYPQFVRS
QTGLGASGAVSH