Protein Info for Psyr_3685 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Silent information regulator protein Sir2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 PF02146: SIR2" amino acids 18 to 195 (178 residues), 121.4 bits, see alignment E=2.2e-39

Best Hits

Swiss-Prot: 93% identical to NPD2_PSESM: NAD-dependent protein deacylase 2 (cobB2) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K12410, NAD-dependent deacetylase [EC: 3.5.1.-] (inferred from 100% identity to psb:Psyr_3685)

Predicted SEED Role

"NAD-dependent protein deacetylase of SIR2 family" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate or Redox-dependent regulation of nucleus processes

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.-

Use Curated BLAST to search for 3.5.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQ55 at UniProt or InterPro

Protein Sequence (247 amino acids)

>Psyr_3685 Silent information regulator protein Sir2 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQTAAALRHAKRILVITGAGLSADSGLPTYRGVGGLYNGKTDDGLPIEMALSGPMLRRDP
ELCWKYIAELGKACLGGEPNVAHYAIAQLQRIKPECWVLTQNVDGYHRAAGSPPERLIEI
HGQLSPLFCQSCGAEDSQLSEHLQRPLPPLCPACSGILRPSVVLFQEMLPEKALETLHEQ
LAKGYDAVLSIGTTASFPYIHEPVIRTRVSGGFTAEINPQPTDHSAQMDVFLPYRAAHVM
AELISHI