Protein Info for Psyr_3523 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Peptidase M20D, amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 TIGR01891: amidohydrolase" amino acids 12 to 368 (357 residues), 360.2 bits, see alignment E=6.4e-112 PF01546: Peptidase_M20" amino acids 69 to 380 (312 residues), 161.5 bits, see alignment E=2.7e-51 PF07687: M20_dimer" amino acids 180 to 275 (96 residues), 45.7 bits, see alignment E=5.8e-16

Best Hits

Swiss-Prot: 45% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to psb:Psyr_3523)

Predicted SEED Role

"Metal-dependent amidase/aminoacylase/carboxypeptidase"

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQL7 at UniProt or InterPro

Protein Sequence (385 amino acids)

>Psyr_3523 Peptidase M20D, amidohydrolase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSISKTLIDEATVWRRTLHAAPELGFQELKTSDKVAELLSGFGIEIHRGLGGTGVVGTLR
NGDGPTIGLRADMDALPIQELGDSVHKSTHKGCMHACGHDGHTAILLATARHLAETRRFR
GTVHFVFQPAEENLGGAQRMIEDGLFERFPMEAIYGLHNWPGVPAGKVVINPGPMMASLD
TFEITLTGKGSHAAMPDKGNDPIVAAAELVLGLQTIVSRRLSPLDSAVVSITQFNAGEAI
NVIPETATLRGTVRCLQTPVREKVQQLIGEFVERLPTAFGVRGELVYNVGYPVTENHVEA
AATIRRAAVAAVGEANVQWGCNPSMASEDFAFMLHACPGAYIWMGVDGEKPSAALHNPYY
DFNDQVIEPGVAVWTSLVEQCLPVA