Protein Info for Psyr_3460 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: PAS/PAC sensor signal transduction histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 PF13188: PAS_8" amino acids 78 to 135 (58 residues), 35.4 bits, see alignment E=1.7e-12 PF00512: HisKA" amino acids 185 to 248 (64 residues), 46.6 bits, see alignment E=7e-16 PF02518: HATPase_c" amino acids 292 to 394 (103 residues), 78.6 bits, see alignment E=1.2e-25 PF13589: HATPase_c_3" amino acids 296 to 345 (50 residues), 26.8 bits, see alignment 1.2e-09

Best Hits

KEGG orthology group: K10942, two-component system, sensor histidine kinase FlrB [EC: 2.7.13.3] (inferred from 100% identity to psb:Psyr_3460)

Predicted SEED Role

"Flagellar sensor histidine kinase FleS" in subsystem Flagellum

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZQT0 at UniProt or InterPro

Protein Sequence (404 amino acids)

>Psyr_3460 PAS/PAC sensor signal transduction histidine kinase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSQAAQLTSALSVQAPYSPELESRQGLEHAFSLFNQMSAQLTDSYGVLEARVTELKGELA
HAGAQRLQELAEKERLANRLQNLLDLLPGGVIVIDGMGVVREANPAAIDLLGQPLLGMLW
RHVISRCFAPREDDGHEVSLKDGRRLSIATRSLDAEPGQLVLLNDLTETRHLQEQLARHE
RLSSLGRMVASLAHQIRTPLSAAMIYASHLTEQELPVETQQRFAARLKDRLHELEHQVRD
MLVFARGELPLTDRLTPGALFQALQNAAATHVQGVSVRWQCDSIDGELLCNRDTLVGALL
NLIENAVQASAGRTLLKIHAYSRGNELRICFSDNGSGIDKAALARIGEPFFTTKTTGTGL
GLAVVTAVSRAHQGRVDYLSRVGRGTCAIVSLPLIPASSPTGNN