Protein Info for Psyr_3374 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Heavy metal response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 PF00072: Response_reg" amino acids 3 to 111 (109 residues), 96.6 bits, see alignment E=1.1e-31 TIGR01387: heavy metal response regulator" amino acids 3 to 218 (216 residues), 337 bits, see alignment E=2.5e-105 PF00486: Trans_reg_C" amino acids 145 to 218 (74 residues), 87.8 bits, see alignment E=4.1e-29

Best Hits

Swiss-Prot: 64% identical to IRLR_BURP2: Transcriptional activator protein IrlR (irlR) from Burkholderia pseudomallei (strain 1026b)

KEGG orthology group: K07665, two-component system, OmpR family, copper resistance phosphate regulon response regulator CusR (inferred from 100% identity to pst:PSPTO_3603)

Predicted SEED Role

"DNA-binding heavy metal response regulator" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZR16 at UniProt or InterPro

Protein Sequence (229 amino acids)

>Psyr_3374 Heavy metal response regulator (Pseudomonas syringae pv. syringae B728a ΔmexB)
MHILLIEDDTKTGEYLKKGLGESGYVVDWTQHGADGLHLALENRYDLIVLDVMLPGIDGW
QIIEVLRARQDVPVLFLTARDQLQDRIRGLELGADDYLVKPFSFTELLLRIRTILRRGVV
READHFHLADLELDLLRRRVTRQQQVIVLTNKEFALLHLLLRREGDVLSRAQIASEVWDM
NFDSDTNVVDVAIKRLRSKVDLPYPVKLIHTVRGIGYVCEVRPCDASLP