Protein Info for Psyr_3242 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Glycerophosphodiester phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01126, glycerophosphoryl diester phosphodiesterase [EC: 3.1.4.46] (inferred from 100% identity to psb:Psyr_3242)

Predicted SEED Role

"Glycerophosphoryl diester phosphodiesterase, periplasmic (EC 3.1.4.46)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (EC 3.1.4.46)

MetaCyc Pathways

glycerol and glycerophosphodiester degradation (4/4 steps found)
glycerophosphodiester degradation (2/2 steps found)
phosphatidylcholine resynthesis via glycerophosphocholine (1/2 steps found)
phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
anandamide biosynthesis I (3/12 steps found)

KEGG Metabolic Maps

Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.46

Use Curated BLAST to search for 3.1.4.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRE8 at UniProt or InterPro

Protein Sequence (380 amino acids)

>Psyr_3242 Glycerophosphodiester phosphodiesterase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQGFPTTLLKKTLLAAAVMSGLMLSSGMAAAADAPGKVLSEKYGLPWPAVIAHRGASFDA
PEETTPAYTLARDLGADYLEMDIQRTKDGVLIALHDDVLERTTNIAQVFPTRVKDPVSTF
TLAELKQLDAGSWFNKAYPDRARDSYNGLQILTLDEVIDIAEGGANKPGLYIETKVPNKF
PGVEADLKKLLAKRGWLEQRPAAAAGHVNVAHMPGRVVLQTFEKQSLELLQKEMPKVPKV
MLLWIGEGSIEPKSSVAFKDSGAKDKASYYATQEVKSPQEFEQWIDWAKAHGAIGTGPSS
QLAKGGDQSYMDLVKPWMNNLTHEKGMVIHPYTVDDAEDFKRISHDGADGFFTNRTAELL
KFYGRPAKESIETILKRNGY