Protein Info for Psyr_3199 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: NADH dehydrogenase subunit D / NADH dehydrogenase subunit C

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 TIGR01961: NADH (or F420H2) dehydrogenase, subunit C" amino acids 43 to 170 (128 residues), 141.6 bits, see alignment E=1.7e-45 PF00329: Complex1_30kDa" amino acids 44 to 172 (129 residues), 151.5 bits, see alignment E=1.6e-48 TIGR01962: NADH dehydrogenase (quinone), D subunit" amino acids 209 to 593 (385 residues), 567.1 bits, see alignment E=1.8e-174 PF00346: Complex1_49kDa" amino acids 323 to 593 (271 residues), 361.9 bits, see alignment E=1.8e-112

Best Hits

Swiss-Prot: 100% identical to NUOCD_PSEU2: NADH-quinone oxidoreductase subunit C/D (nuoC) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K13378, NADH dehydrogenase I subunit C/D [EC: 1.6.5.3] (inferred from 100% identity to psb:Psyr_3199)

MetaCyc: 93% identical to NADH-quinone oxidoreductase subunit C/D (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRJ1 at UniProt or InterPro

Protein Sequence (593 amino acids)

>Psyr_3199 NADH dehydrogenase subunit D / NADH dehydrogenase subunit C (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTADNALYIPPYKADDQDVVVELNNRFGAEAFTAQPTRTGMPVLWVERARLFEILTFLRN
VPKPYSMLYDLHGVDERLRTNRRGLPGADFTVFYHLLSIERNSDVMIKVALSESDLSVPT
ITSIWPNANWYEREVWDMFGIDFPGHPHLSRIMMPPTWEGHPLRKDFPARATEFDPYSLT
LAKQQLEEEAARFRPEDWGMKRSGANEDYMFLNLGPNHPSAHGAFRIILQLDGEEIVDCV
PDIGYHHRGAEKMAERQSWHSFIPYTDRIDYLGGVMNNLPYVLSVEKLAGIKVPEKVDVI
RIMMAEFFRITSHLLFLGTYIQDVGAMTPVFFTFTDRQKAYTVIEAITGFRLHPAWYRIG
GVAHDLPRGWEKLVKDFVDWLPKRLDEYTKAALQNSILKGRTIGVAAYNTKEALEWGVTG
AGLRSTGCDFDLRKARPYSGYENFEFEVPLAANGDAYDRCMVRVEEMRQSIKIIDQCMRN
MPEGPYKADHPLTTPPPKERTLQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYY
LTSDGGTMSYRTRIRTPSYPHLQQIPSVIKGSMVADLIAYLGSIDFVMADVDR