Protein Info for Psyr_3183 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 740 (738 residues), 1098.1 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 54.9 bits, see alignment 6.7e-18 PF13191: AAA_16" amino acids 192 to 251 (60 residues), 34.7 bits, see alignment 2.2e-11 PF05621: TniB" amino acids 200 to 352 (153 residues), 23 bits, see alignment E=4.2e-08 PF00004: AAA" amino acids 216 to 347 (132 residues), 48 bits, see alignment E=1.5e-15 amino acids 497 to 612 (116 residues), 50.6 bits, see alignment E=2.4e-16 PF17871: AAA_lid_9" amino acids 355 to 457 (103 residues), 95.1 bits, see alignment E=1.9e-30 PF07724: AAA_2" amino acids 491 to 652 (162 residues), 208.2 bits, see alignment E=7.5e-65 PF00158: Sigma54_activat" amino acids 495 to 618 (124 residues), 24.4 bits, see alignment E=1.8e-08 PF13401: AAA_22" amino acids 495 to 587 (93 residues), 27.1 bits, see alignment E=3.9e-09 PF07728: AAA_5" amino acids 496 to 614 (119 residues), 53.1 bits, see alignment E=2.9e-17 PF10431: ClpB_D2-small" amino acids 658 to 738 (81 residues), 91.1 bits, see alignment E=3.2e-29

Best Hits

Swiss-Prot: 66% identical to CLPA_ECOLI: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli (strain K12)

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 100% identity to psb:Psyr_3183)

MetaCyc: 66% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRK7 at UniProt or InterPro

Protein Sequence (757 amino acids)

>Psyr_3183 ATP-dependent Clp protease ATP-binding subunit ClpA (Pseudomonas syringae pv. syringae B728a ΔmexB)
MLNRELEVTLNLAFKEARSKRHEFMTVEHLLLALLDNEAAATVLRACGANLDKLKHDLQE
FIDSTTPLIPVHDEDRETQPTLGFQRVLQRAVFHVQSSGKREVTGANVLVAIFSEQESQA
VFLLKQQSVARIDVVNYIAHGISKVPGHGEHSSEGEQDMQDDEGGEASSSGNPLDAYASN
LNELARQGRIDPLVGRESEVERVAQILARRRKNNPLLVGEAGVGKTAIAEGLAKRIVDNQ
VPDLLANSVVYSLDLGALLAGTKYRGDFEKRFKALLNELRKRPQAILFIDEIHTIIGAGA
ASGGVMDASNLLKPLLSSGEIRCIGSTTFQEFRGIFEKDRALARRFQKVDVSEPSVEDTI
GILRGLKSRFEQHHSIEYSDEALRAAAELASRYINDRHMPDKAIDVIDEAGAYQRLQPVE
KRAKRIDVPEVEDIVAKIARIPPKHVNSSDKELLRNLERDLKLTVFGQDAAIDSLSTAIK
LSRAGLKSPDKPVGSFLFAGPTGVGKTEAARQLAKALGVELIRFDMSEYMERHTVSRLIG
APPGYVGFDQGGLLTEAITRQPHCVLLLDEIEKAHPEVFNLLLQVMDHGTLTDNNGRKAD
FRNVIVIMTTNAGAESAARASIGFTHQDHSSDAMEVIKKSFTPEFRNRLDTIIQFGRLSH
EVIKSVVDKFLTELQAQLEDKRVLLEVSEAARSWLAQGGYDAAMGARPMARLIQDKIKRP
LAEEILFGELSEHGGVVHIDIKDGEITFDFETTAEMA