Protein Info for Psyr_3076 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Type I secretion system ATPase, HlyB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 720 transmembrane" amino acids 165 to 185 (21 residues), see Phobius details amino acids 192 to 212 (21 residues), see Phobius details amino acids 272 to 297 (26 residues), see Phobius details amino acids 303 to 323 (21 residues), see Phobius details PF03412: Peptidase_C39" amino acids 18 to 136 (119 residues), 74.7 bits, see alignment E=9.7e-25 TIGR01846: type I secretion system ATPase" amino acids 20 to 715 (696 residues), 1042.1 bits, see alignment E=0 PF00664: ABC_membrane" amino acids 165 to 426 (262 residues), 206.7 bits, see alignment E=8.7e-65 PF00005: ABC_tran" amino acids 494 to 643 (150 residues), 115.2 bits, see alignment E=5.4e-37

Best Hits

Swiss-Prot: 60% identical to LKB2A_MANHA: Leukotoxin translocation ATP-binding protein LktB (lktB) from Mannheimia haemolytica

KEGG orthology group: K11004, ATP-binding cassette, subfamily B, bacterial HlyB/CyaB (inferred from 100% identity to psb:Psyr_3076)

Predicted SEED Role

"cyclolysin secretion ATP-binding protein" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZRW2 at UniProt or InterPro

Protein Sequence (720 amino acids)

>Psyr_3076 Type I secretion system ATPase, HlyB (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNDSEMGTQMPDNAPRLDTGLASLVMLARFHQVAASPEQLAHEFGSPDQYLSQDSLLLAA
RKLGLKAKAARTTTERLDRTPLPAIAADNNGGFFIIARLDQGKALIHDPFSQRPEVVTFE
ALQERWTGQLLLIRSEAGTPGESSSFDFTWFIPAIVKYRKLLGEVMLVSFVLQIFSLITP
LFFQVVMDKVLVHHGLTTLDVIAIGLAGVMLFESALSGLRTYVFAHTASRIDVELGSKLF
RHLITLPLSYFQARRVGDSVARVRELENIRNFLTGNAITLLLDVLFSVVFIAVMFYYSGW
LTLIVLLSLPLYILVSVLITPVLRKRLNDSFARGAENQAFLVETVNGIDTLKSMAVEPQA
IRKWDNQLAGYVAAGFKTQNLSTIANESVSLIGKLVTVATLWFGARLVIDGQLSVGQLIA
FNMLAGRVAQPIMRLAQLWTNFQQTGVSVQRLGDILNTRNELSQVNRSALPPIKGRIEFD
QVHFRYRPDGSEILRGINLVITPGQVIGVVGRSGSGKSTLTRLLQRLYVPERGRVLVDGM
DLALADVSSLRRQIGVVLQDNMLFNRSIRENIALSDAGAPLEAVMQMAKLAGAHDFILEL
PEGYDTMVGEHGASLSGGQRQRIAIARALMGNPRILIFDEATSALDYESERIVQQNMQAI
CKGRTVIIIAHRLSAVRDANRIVVVDRGQIVEQGTHAELLTHQAGHYSRLHRLQQGGSHV