Protein Info for Psyr_2486 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 transmembrane" amino acids 6 to 29 (24 residues), see Phobius details amino acids 41 to 67 (27 residues), see Phobius details amino acids 76 to 97 (22 residues), see Phobius details amino acids 106 to 126 (21 residues), see Phobius details amino acids 140 to 160 (21 residues), see Phobius details amino acids 171 to 192 (22 residues), see Phobius details amino acids 219 to 237 (19 residues), see Phobius details PF03707: MHYT" amino acids 52 to 108 (57 residues), 69.3 bits, see alignment 3.5e-23 amino acids 115 to 170 (56 residues), 65.5 bits, see alignment 5.3e-22 amino acids 182 to 233 (52 residues), 24.4 bits, see alignment 3.5e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 259 to 421 (163 residues), 141.8 bits, see alignment E=8.7e-46 PF00990: GGDEF" amino acids 263 to 418 (156 residues), 157.1 bits, see alignment E=5.2e-50 PF00563: EAL" amino acids 439 to 672 (234 residues), 247.7 bits, see alignment E=1.6e-77

Best Hits

Swiss-Prot: 60% identical to Y1727_PSEAE: Uncharacterized signaling protein PA1727 (PA1727) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2486)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZTJ7 at UniProt or InterPro

Protein Sequence (703 amino acids)

>Psyr_2486 diguanylate cyclase/phosphodiesterase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MLIGSYIPSLVVISILVAILAAYTALDLVGRIVSARGRAVHLWIAGGAVAMGVGTWSTHF
IGMLAFVLPIDLGYDVPLVLLSLLIAIGFSGFALWLATLPRLPALQLSLGSLLLGLGISA
THYTGMAAMRMQPGIQYTPWLFALSLVTAVVASAAALRVAFHLRQQRPRVYVLRASAALL
LGLAVIGMHYTGMAAANFADGSFCGALPQGLSSNGLDRFVLVASLSILGIALFSCILDSH
LETRTAVLADSLTQANLELTHLALHDNLTGLPNRALLTERIDQAMKRATETGGCFALMFM
DLDGFKPVNDAFGHHTGDLLLRQVALRLRNSLHRRDTLARVGGDEFVLLVELQHPEDALA
VARRQVNEVGNPFIIGEHQLQISLSIGICVYPGNGSTQHELLINADAAMYHTKAAGKNGY
SFFDVSMNSNARNQLQMSQDLHKAIKHRQFCIYYQPKFDAMTGLPVGAEALLRWNHPKQG
VLGPDLFISMAEKTGLIIQIGEWVLDEACRQMREWYTQGYSHWRIAVNLSALQFCHAGLV
TAVADTLARHQLPANCLTLEITETTAMHDADASLAVLRRLSEMGVDLSIDDFGTGYSSLM
YLKRLPANEIKIDRGFVRDLEHDTDDAAIVSAIVAVGQALNLRIVAEGVETAVQQRFLTH
LGCHSLQGFLLGYPLPAEQFLEEIRAAEAKAAVEASDTGQAAF