Protein Info for Psyr_2314 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: pyruvate dehydrogenase (cytochrome)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02776: TPP_enzyme_N
  • amino acids 33 to 144 (112 residues), 108.9 bits, E=2.1e-35
• PF00205: TPP_enzyme_M
  • amino acids 220 to 347 (128 residues), 112.9 bits, E=1.4e-36
• PF02775: TPP_enzyme_C
  • amino acids 409 to 555 (147 residues), 137.4 bits, E=5.1e-44

Best Hits

Swiss-Prot: 66% identical to POXB_ECOLI: Pyruvate dehydrogenase [ubiquinone] (poxB) from Escherichia coli (strain K12)

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 100% identity to psb:Psyr_2314)

MetaCyc: 66% identical to pyruvate oxidase (Escherichia coli K-12 substr. MG1655)
RXN-11496 [EC: 1.2.5.1]; RXN0-2022 [EC: 1.2.5.1]

Predicted SEED Role

"Pyruvate oxidase [ubiquinone, cytochrome] (EC 1.2.2.2)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.2.2)

MetaCyc Pathways

• pyruvate to cytochrome bo oxidase electron transfer (2/2 steps found)
• pyruvate to cytochrome bd oxidase electron transfer (1/2 steps found)
• pyruvate fermentation to acetoin (1/3 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.2.2 or 1.2.5.1

Sequence Analysis Tools

See Q4ZU15 at UniProt or InterPro

Protein Sequence (601 amino acids)

>Psyr_2314 pyruvate dehydrogenase (cytochrome) (Pseudomonas syringae pv. syringae B728a ΔmexB)
MHWVDHSRRGRALRAFYRTHYHCLDKEPRMSKTIADHLAQTLAAAGVSHIWGVSGDSLNG
LTDSLERTDSIRWMHTRHEEVAAFAAGAQAASSGKLAVCAGSCGPGNLHLINGLYDCHRN
RVPVLAIAAHIPSSEIGLDYFQETHPQELFKECSHFVELVSNPEQFPRVLERAMRAAISQ
KGVAVIVLPGDVALSDAPDVAAKWIEATAPKVVPAENDLQSMADMLNASKAVTLLCGAGC
EGAHEQLLALADTLGAPIVHALRGKQHVEYDNPFDVGMTGLIGFSSGYHAMLSCDTLVIL
GSSFPYRNFYPEKADIIQIDLDPTQIGRRTPVALGLVGGVRDTLEALQPKLKPHTDRRFL
DKALKHYAKAREELDELATPTPDGAPIHPQYLTRLVDEQADADAIFTVDVGTPTLWAARY
LHMNGKRSLLGSFNHGSMANALPQALGAKAEHPDRQVIALCGDGGLSMLLGDLLSIRQLN
LPIKMVVFNNSSLGFVDMEMKASGYVPHGTDLHETNFAGIALGAGILGLRVENAEELPAA
LRKAFDHPGPVLIDVVTAKQELGIPPKIKLAQARGFSLYMMRAIMSGRGSEVLELAKTNL
R