Protein Info for Psyr_2261 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Peptidase M20:Peptidase M20

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 PF01546: Peptidase_M20" amino acids 82 to 378 (297 residues), 88.8 bits, see alignment E=5e-29 PF07687: M20_dimer" amino acids 184 to 283 (100 residues), 72.4 bits, see alignment E=2.8e-24

Best Hits

KEGG orthology group: K01295, glutamate carboxypeptidase [EC: 3.4.17.11] (inferred from 100% identity to psb:Psyr_2261)

Predicted SEED Role

"Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases"

Isozymes

Compare fitness of predicted isozymes for: 3.4.17.11

Use Curated BLAST to search for 3.4.17.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZU68 at UniProt or InterPro

Protein Sequence (383 amino acids)

>Psyr_2261 Peptidase M20:Peptidase M20 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNEAQLHASAWLAEQYEAMEALLQRLVDTDSNSYDKVGVDAVGELLVEQLQVDGIDVTRI
PVEGFGDVLLAELPGGPGKPVLLLGHRDTVFPKGTTSTRGYTRDAELAYGPGVADMKGGL
VLNCFALKALKRAGPLPFPVQILYTGDEEIGSASARNHIEHYARQARAVLNPEPGRASGN
VVSARKGGATLIIEVSGRAAHSGVNHADGASAIQALAHKIIKLHALTDYAAGITTNVGLI
SGGTSSNTVAPSATAKLDVRFVELRQWAEILAAVQAIVAEEELSGTSARLLEATTFLPME
ARHSTELLSIYQGLAQELGFSVEGEFTGGCADSGFTASLGIPTLCGLGPVGGKVHTDREY
LELNTLVPRGQALVATILALGDV