Protein Info for Psyr_2209 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: ABC transporter, substrate-binding protein, aliphatic sulfonate

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF13379: NMT1_2" amino acids 35 to 261 (227 residues), 27.3 bits, see alignment E=7.9e-10 TIGR01728: ABC transporter, substrate-binding protein, aliphatic sulfonates family" amino acids 38 to 319 (282 residues), 199.2 bits, see alignment E=4.2e-63 PF04069: OpuAC" amino acids 56 to 240 (185 residues), 29.7 bits, see alignment E=1.3e-10 PF12974: Phosphonate-bd" amino acids 81 to 203 (123 residues), 27.9 bits, see alignment E=4.2e-10 PF09084: NMT1" amino acids 82 to 259 (178 residues), 47.8 bits, see alignment E=4.3e-16 PF00497: SBP_bac_3" amino acids 101 to 245 (145 residues), 33 bits, see alignment E=1.2e-11

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 100% identity to psb:Psyr_2209)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUC0 at UniProt or InterPro

Protein Sequence (324 amino acids)

>Psyr_2209 ABC transporter, substrate-binding protein, aliphatic sulfonate (Pseudomonas syringae pv. syringae B728a ΔmexB)
MPKPIKHTQTDYRRALLAGLLAGLALCITLPAEAQETLRIGYQKSSTLITLLKTQGTLEK
ALAAQHVAVTWHEFPSGLPLLEALNVGNVDISADVADTVQIFAQAAQARLTYFAQEAASP
SAQAIIVHKNSPVRQLWDLKGKKVAVTKAAGSHYLLIAALKKAGLAFSDIQPAYLSPADG
RAAFENNKVDAWVTWEPFLTSAQRQLPTRTLADGKGLASYKRYYLTGTGYAKEHPQVLSV
VYEQLHKAGIWLKANPRDAAKVLSPLWGNLDIETVEIANSNRAYQIQPVTHDQLDEQQHI
ADAFFAAGLLPKAVDAQDVEVWKP