Protein Info for Psyr_2110 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: diguanylate cyclase with PAS/PAC sensor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 315 to 334 (20 residues), see Phobius details PF03924: CHASE" amino acids 88 to 269 (182 residues), 124.8 bits, see alignment E=1.1e-39 PF00989: PAS" amino acids 365 to 491 (127 residues), 33.5 bits, see alignment E=1.1e-11 TIGR00229: PAS domain S-box protein" amino acids 373 to 501 (129 residues), 48.7 bits, see alignment E=7.8e-17 PF13426: PAS_9" amino acids 376 to 493 (118 residues), 37.7 bits, see alignment E=6.4e-13 PF08447: PAS_3" amino acids 534 to 617 (84 residues), 35.4 bits, see alignment E=3.1e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 633 to 792 (160 residues), 161.2 bits, see alignment E=1.8e-51 PF00990: GGDEF" amino acids 637 to 790 (154 residues), 147.4 bits, see alignment E=9.5e-47

Best Hits

KEGG orthology group: None (inferred from 100% identity to psb:Psyr_2110)

Predicted SEED Role

"CHASE domain/PAS domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZUL9 at UniProt or InterPro

Protein Sequence (796 amino acids)

>Psyr_2110 diguanylate cyclase with PAS/PAC sensor (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAE
RFSRLQERLDRQTTRLDTLARFFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERT
AFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFIQTLSDIPTPAGFDIASEPVR
RAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQ
LMNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPT
QIFSLNNQIMMPGRVLWLGGLLSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRA
AHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYAQEDVLGRFRLMDFYQSADLE
ARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGL
WTGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMC
ELFEVDEEQLLGDSETILQRIQPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLR
SASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDVLTGAYNRRYFQDRMQAELKR
IDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFM
VICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGV
YAAKQAGRDRVEPEQG