Protein Info for Psyr_1969 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: ABC transporter:Protein of unknown function DUF214

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 657 transmembrane" amino acids 285 to 305 (21 residues), see Phobius details amino acids 533 to 557 (25 residues), see Phobius details amino acids 587 to 610 (24 residues), see Phobius details amino acids 616 to 639 (24 residues), see Phobius details PF00005: ABC_tran" amino acids 26 to 174 (149 residues), 109.6 bits, see alignment E=3e-35 PF12704: MacB_PCD" amino acids 284 to 501 (218 residues), 165.3 bits, see alignment E=3.5e-52 PF02687: FtsX" amino acids 538 to 650 (113 residues), 63.3 bits, see alignment E=3.2e-21

Best Hits

Swiss-Prot: 100% identical to MACB1_PSEU2: Macrolide export ATP-binding/permease protein MacB 1 (macB1) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K05685, macrolide transport system ATP-binding/permease protein [EC: 3.6.3.-] (inferred from 100% identity to psb:Psyr_1969)

Predicted SEED Role

"Pyoverdine efflux carrier and ATP binding protein" in subsystem Siderophore Pyoverdine

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZV10 at UniProt or InterPro

Protein Sequence (657 amino acids)

>Psyr_1969 ABC transporter:Protein of unknown function DUF214 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MRTPLIDLRGIRKSYGGGDSPLVNVLRGIDLSIHTGEFVAIVGASGSGKSTLMNILGCLD
RPTSGEYLFAGENVAALGSDELAWLRREAFGFVFQGYHLIPSGSAQENVEMPAIYAGTPA
AERHARAAALLDRLGLASRTGNRPHQLSGGQQQRVSIARALMNGGHIILADEPTGALDSH
SGAEVMTLLDELASQGHVVILITHDREVAARAKRVIEISDGLVISDTAQDASDVQRSVNP
AALQAVDLRKRLSEGSGSQSAWQGELFDAIRAAWRVMWINRFRTALTLLGIVIGVASVVV
MLAVGEGSKRQVMAQMSSFGSNIIYLNGKAPNPRAPKGVITLEEVAALGELPEVKMIMPV
NGGQAGVRYGNLDHSSYVGGNDTHFPAIFNWPVVEGSYFSEADEQNAAAVAVIGYKVRQK
LFGEQSDPVGQYILIENVPFQVVGVLQEKGATSGDLDSDNRIAIPYSSASIRLFGTQDPE
YITIATRDANNVKQAEQSIRNLLQQLHHGKQDYELTNNAAMIQAEARTQNTLSLMLGSIA
AISLLVGGIGVMNIMLMTVRERTREIGIRMATGARQSDILRQFLTEAVMLSVVGGLAGIV
LALGMGAALLLSKVAVAFTLPAVAGAFACALITGVIFGFMPARKAARLDPVAALTSE