Protein Info for Psyr_1731 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Drug resistance transporter EmrB/QacA subfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 54 to 74 (21 residues), see Phobius details amino acids 83 to 103 (21 residues), see Phobius details amino acids 112 to 133 (22 residues), see Phobius details amino acids 141 to 163 (23 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 203 to 222 (20 residues), see Phobius details amino acids 234 to 253 (20 residues), see Phobius details amino acids 274 to 293 (20 residues), see Phobius details amino acids 305 to 325 (21 residues), see Phobius details amino acids 336 to 356 (21 residues), see Phobius details amino acids 368 to 389 (22 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details amino acids 478 to 496 (19 residues), see Phobius details PF06609: TRI12" amino acids 12 to 270 (259 residues), 32.8 bits, see alignment E=4.2e-12 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 15 to 494 (480 residues), 515.4 bits, see alignment E=7.9e-159 PF07690: MFS_1" amino acids 21 to 415 (395 residues), 183 bits, see alignment E=1.2e-57 PF00083: Sugar_tr" amino acids 36 to 186 (151 residues), 39 bits, see alignment E=7.3e-14

Best Hits

Swiss-Prot: 51% identical to EMRB_ECO57: Multidrug export protein EmrB (emrB) from Escherichia coli O157:H7

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to psb:Psyr_1731)

MetaCyc: 51% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZVP3 at UniProt or InterPro

Protein Sequence (512 amino acids)

>Psyr_1731 Drug resistance transporter EmrB/QacA subfamily (Pseudomonas syringae pv. syringae B728a ΔmexB)
MSNNAPASFTPPSLLLCTIGLSLATFMQVLDTTIANVALPTIAGNLGVSSEQSTWVITSF
AVSNAIALPLTGWLSRRFGEVKLFLWATILFVIASFLCGISQSMPELVGFRALQGMVAGP
LYPMSQTLLIAVYPPAKRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINIPIG
LFAVLVVRSQMSKRPVTTARQPLDYIGLLALIVGVGALQVVLDKGNDLDWFESNFIIFGS
LISLVALVFFVIWEMTDKHPIVNLRLFAYRNFRIGTLVMIGGYSGFFGINLILPQWLQTQ
MGYTATWAGLAVAPIGILPVLMSPFVGKYAHKFDLRLLAGLAFLAMGLSCFMRAGFNTDV
DFEHVAMVQLFMGIGVALFFMPTLSILLSDLPPDQIADGSGLATFLRTLGGSFAASLTTW
IWIRRANQHHAYLSENISTFDPATRHALDSLGGASPQAYAQLEQTLNAQAYMMSTVDYFY
LLGWIFAGLILLVWLAKPPFAAKAGPAAGGGH