Protein Info for Psyr_1730 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Secretion protein HlyD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 transmembrane" amino acids 25 to 45 (21 residues), see Phobius details PF00529: CusB_dom_1" amino acids 40 to 348 (309 residues), 46.2 bits, see alignment E=7.3e-16 PF13533: Biotin_lipoyl_2" amino acids 64 to 111 (48 residues), 29.4 bits, see alignment 1.1e-10 PF16576: HlyD_D23" amino acids 204 to 294 (91 residues), 49.6 bits, see alignment E=6.3e-17 PF13437: HlyD_3" amino acids 220 to 299 (80 residues), 49.3 bits, see alignment E=1.4e-16

Best Hits

Swiss-Prot: 43% identical to EMRA_HAEIN: Multidrug export protein EmrA (emrA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to psb:Psyr_1730)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZVP4 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Psyr_1730 Secretion protein HlyD (Pseudomonas syringae pv. syringae B728a ΔmexB)
MATAAAENTSAPAPEQEPKSGKRTFMLIGLAIVVVILALGIWAWYEIYGQWSEETDDAYV
NGNVVEITPLVTGTVISIGADDGDLVHEGQVLLKFDPSDAEVSLQSAEANLGKVVRQVRG
LYSNVDGMKAQLAAQRAAVQTAQDNYNRRRSLAAGGAISQEELSHARDSLTSAQSALNNI
QQQLSTSVALVDDTVVSSHPDVKAAAAQLRQAYLTNARSTLVAPVTGYVAKRTVQLGQRI
QPGTATMAVIPLDQLWIDANFKETQLGKMRIGQPVEISSDLYGSDVKYSGTIDSLGAGTG
SAFALLPAQNATGNWIKIVQRVPVRVHINPEELAKYPLRIGLSTTVEVNLHDQSGPVLAQ
QPRKQAAFSTQVYTEQLADADALIARLIHENSAPGGKTAVQR