Protein Info for Psyr_1690 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Peptidase M1, membrane alanine aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 888 TIGR02414: aminopeptidase N" amino acids 13 to 886 (874 residues), 1327.1 bits, see alignment E=0 PF17900: Peptidase_M1_N" amino acids 56 to 192 (137 residues), 57.6 bits, see alignment E=3.7e-19 PF01433: Peptidase_M1" amino acids 232 to 445 (214 residues), 185.2 bits, see alignment E=2.7e-58 PF11940: DUF3458" amino acids 450 to 556 (107 residues), 104.6 bits, see alignment E=6.2e-34 PF17432: DUF3458_C" amino acids 561 to 887 (327 residues), 405.5 bits, see alignment E=3.8e-125

Best Hits

KEGG orthology group: K01256, aminopeptidase N [EC: 3.4.11.2] (inferred from 100% identity to psb:Psyr_1690)

Predicted SEED Role

"Membrane alanine aminopeptidase N (EC 3.4.11.2)" (EC 3.4.11.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.11.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZVT4 at UniProt or InterPro

Protein Sequence (888 amino acids)

>Psyr_1690 Peptidase M1, membrane alanine aminopeptidase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MRTEQPKMIYLKDYQAPEYLIDETNLTFELFDDHSLVHAQLVMRRNPERGAGLPALVLDG
QNLELISVKLSDVELGAADYQLTDDHLTLHPQADSFTVDSTVRIHPESNTALEGLYKSSG
MFCTQCEAEGFRKITYYLDRPDVMSKFTTTVSADKQNFPILLSNGNPVASGEEEDGRHWA
TWEDPFMKPAYLFALVAGDLWCIEDTFTTMNERNVTLRIYVEPENIDKCQHAMTSLKKSM
RWDEETYGREYDLDIFMIVAVNDFNMGAMENKGLNIFNSSAVLARAETATDAAHQRVEAI
VAHEYFHNWSGNRVTCRDWFQLSLKEGFTVYRDAGFSADMNSATVKRIQDVAYLRTHQFA
EDAGPMAHAVRPDSFIEISNFYTLTVYEKGSEVVGMLHTLLGAEGFRKGSDLYFERHDGQ
AVTCDDFIKAMEDANGVDLTQFKRWYSQAGTPRLAVSESYDAAAKTYSLTFRQSCPTTPG
QPGDQKQPFVIPVELGLLDSKGGEIALRLANETAATGTTRVLSVTEAEQTFTFVDIAEKP
LPSLLRGFSAPVKLSFPYDRDQLMFLMQHDSDGFNRWDAGQQLSVQVLQELIGQHQQGQA
LLMDQRLITALGTVLADDGLDQAMVAEMLSLPGEAYLTEISEVADVDAIHAAREFARQQL
AEGLFDGLLARYQANREVSKVTPYVAEAAHFARRALQNIALSYLMLSGKPEVLAATIDQF
DTSDNMTERLTALAVLVNSPFTAERDKALDVFAENFKSNALVMDQWFSVQAASTLPGGLQ
RVQELMKHPAFSIKNPNKVRALIGAFAGQNLVNFHAADGSGYRFLADLVIELNALNPQIA
SRQLAPLTRWRKYDSARQARMKAELERILASGKLSADVYEVVSKSLAE