Protein Info for Psyr_1361 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: CTP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00337: CTP synthase
  • amino acids 2 to 534 (533 residues), 835 bits, E=1.2e-255
• PF06418: CTP_synth_N
  • amino acids 3 to 265 (263 residues), 419.4 bits, E=9e-130
• PF00117: GATase
  • amino acids 300 to 533 (234 residues), 142.8 bits, E=1.6e-45
• PF07722: Peptidase_C26
  • amino acids 364 to 516 (153 residues), 28.4 bits, E=2e-10

Best Hits

Swiss-Prot: 100% identical to PYRG_PSEU2: CTP synthase (pyrG) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01937, CTP synthase [EC: 6.3.4.2] (inferred from 100% identity to psb:Psyr_1361)

MetaCyc: 70% identical to CTP synthetase (Escherichia coli K-12 substr. MG1655)
CTP synthase. [EC: 6.3.4.2]

Predicted SEED Role

"CTP synthase (EC 6.3.4.2)" (EC 6.3.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• superpathway of pyrimidine nucleobases salvage (4/4 steps found)
• UTP and CTP de novo biosynthesis (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)
• superpathway of pyrimidine ribonucleosides salvage (6/10 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.2

Use Curated BLAST to search for 6.3.4.2

Sequence Analysis Tools

See Q4ZWR0 at UniProt or InterPro

Protein Sequence (543 amino acids)

>Psyr_1361 CTP synthase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVF
VTHDGAETDLDLGHYERFIRTTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITD
EIKRRIIKGAGDADVALVEIGGTVGDIESQPFLEAIRQLRFEVGARRAMLMHLTLVPYIA
TAGETKTKPTQHSVKELRSIGLQPDVLVCRSDHPIDVSSRRKIAQFTNVEERAVIALEDA
DTIYKIPGILHSQGLDDFVVERFGLQCGGADLSEWDKVVDAKLNPEHEVTIAMVGKYMEL
LDAYKSLIEAMSHAGITNRTKVNLRYIDSEDIENQGTGLLEGVDAILVPGGFGLRGVEGK
ITAVQFARENKVPYLGICLGMQVAVIEFARNVLGWKDANSTEFDRTSAHAVVGLITEWED
ATGAVETRTESSDLGGTMRLGAQECQLEAGSLVHDCYTKDVIVERHRHRYEVNNNLLPQL
IEAGLKISGRSGDGALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQ
KNS