Protein Info for Psyr_1292 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: multi-sensor hybrid histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1209 signal peptide" amino acids 1 to 38 (38 residues), see Phobius details transmembrane" amino acids 540 to 559 (20 residues), see Phobius details PF00497: SBP_bac_3" amino acids 75 to 283 (209 residues), 42.3 bits, see alignment E=2.6e-14 amino acids 313 to 525 (213 residues), 58 bits, see alignment E=3.4e-19 PF00989: PAS" amino acids 583 to 693 (111 residues), 33.4 bits, see alignment E=1.6e-11 PF13188: PAS_8" amino acids 584 to 633 (50 residues), 20.4 bits, see alignment (E = 1.5e-07) TIGR00229: PAS domain S-box protein" amino acids 585 to 703 (119 residues), 43.4 bits, see alignment E=1.8e-15 PF08448: PAS_4" amino acids 587 to 698 (112 residues), 48.8 bits, see alignment E=2.9e-16 PF00512: HisKA" amino acids 716 to 783 (68 residues), 52.6 bits, see alignment 1.5e-17 PF02518: HATPase_c" amino acids 831 to 945 (115 residues), 100.5 bits, see alignment E=3e-32 PF00072: Response_reg" amino acids 970 to 1083 (114 residues), 81.7 bits, see alignment E=1.7e-26 PF01627: Hpt" amino acids 1111 to 1191 (81 residues), 36 bits, see alignment 2.7e-12

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 100% identity to psb:Psyr_1292)

Predicted SEED Role

"Signal transduction histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZWX9 at UniProt or InterPro

Protein Sequence (1209 amino acids)

>Psyr_1292 multi-sensor hybrid histidine kinase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQSVVRETGSTSRCRAWRRVWMLLALVFAGAANAAQSLPFSLTAPFVASADMVLEDQDRA
WLDQRKVLQVGIAIADYEPIDITSDRNRYQGISADYLGLVSDQLNLPVQVTGFAKRDEAI
EALRDGKIDLLTSANGFERGVKGLSFSTEYMPDRSVVVGRGNDLSPSSSLAGKKVVLLDG
YADSDVLHRIYPDSQIIIAPNLYSALEALSQGEVDVFIGNEVIVRTYTALRPYLGLQIKF
ESRLPPVGFSFAVRDDEQRLLNFINRALDSIAPSTSREVLGRWTMGLGADVEGQRIRLTS
AERRWLLKHPSVTIATVQHPPYIYKDNNGHWVGLNADVLSRISRMTGLQFVHQESLSTQQ
SIDMLRAGQADMNTTLAENTERRRFLDFTYSFGGNSWMFVVRSDHSSRISLDSLAGKVLA
LPARHALEDLIRREHPLIQLRLVNTYDQARALVESGAADATIQNEVGAYLFPSGELKVAR
SVEGQWSPDRFSVIKTQPELLGILNKALEEFPVAELRSIRLKWLGSALPQPSLWARIPPW
VFWVVSLALLTGLVSLTWSSRLKVQIRQRQRAQRQLNDQLAFKHALLDGIPNPIYVRDLK
GRLISCNRSYEQSLGISFEQMNGRRLTDVNLIPRALAEQMHTDYLNLLENHQPVFSDRTI
ELPGKRMDVWQWTVPFFAADGQLQGLLGGWIDITERKQLEQQLQEATRLAAQANEAKSAF
LASMSHEIRTPMGAIIGLLELECEQALRLGTMPSQGLQVAHRSATELVALIGESLDLARI
EAGGMQLSLAVTSLQGLFDGVIELFSAQAREKGVELRLEFSAQARGDYWLDPLRLRQVLH
NVLGNALKFTRQGAVVVRLDVTSDSPESTRVRIGIQDSGEGIDPQRQQQVFQPFTQANDD
TAAHYGGSGLGLSITRQLVELMRGDISLHSEPGKGTLVTIDLPLTRVSEPVLPADDVTDA
PVDTRSLHLLVVDDMSANRLVLTRQLEFLGHQVTAVEDGKAALSNWCEGAFDAVITDCNM
PGISGYALTQAIRQIEEKEQRQRCPVIGCTANAMSDEAARCEQAGMDGLLIKPLSLARLA
QELADRVREPTFDIGTLQAMTQANPQQMQRLLSELWKNLRHEHALLEPAVSANDWKTLGA
CLHRLKGAASLVDAVPLAKACAALDDSVRLQSAARLAERWQVLEVAMTGLRADIELQLVA
VPETAVPGA