Protein Info for Psyr_1245 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: 23S rRNA m(2)A-2503 methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR00048: 23S rRNA (adenine(2503)-C(2))-methyltransferase" amino acids 3 to 358 (356 residues), 480.7 bits, see alignment E=1.3e-148 PF21016: RlmN_N" amino acids 8 to 67 (60 residues), 106 bits, see alignment E=6.7e-35 PF04055: Radical_SAM" amino acids 111 to 285 (175 residues), 62.4 bits, see alignment E=6.4e-21

Best Hits

Swiss-Prot: 100% identical to RLMN_PSEU2: Dual-specificity RNA methyltransferase RlmN (rlmN) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K06941, ribosomal RNA large subunit methyltransferase N [EC: 2.1.1.-] (inferred from 100% identity to psb:Psyr_1245)

MetaCyc: 60% identical to 23S rRNA m2A2503 methyltransferase/tRNA m2A37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11586 [EC: 2.1.1.192]; 2.1.1.- [EC: 2.1.1.192]

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase N (EC 2.1.1.-)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.192

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZX26 at UniProt or InterPro

Protein Sequence (382 amino acids)

>Psyr_1245 23S rRNA m(2)A-2503 methyltransferase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MIASTGKTNLLGLTQQEMEKFFDSIGEKRFRAGQVMKWIHHFGVDDFDAMTNVSKALREK
LKACAEVRGPEVVSEDISSDGTRKWVVRVESGSCVETVYIPQGKRGTLCVSSQAGCALDC
SFCSTGKQGFNSNLTAAEVIGQVWIANKSFGSVPATVDRAITNVVMMGMGEPLLNFDNVI
AAMHLMMDDLGYGISKRRVTLSTSGVVPMIDELSKHIDVSLALSLHAPNDALRNQLVPIN
KKYPLKMLLESCRRYMSSLGEKRVLTIEYTMLKDINDKVEHAVEMIELLKDTPCKINLIP
FNPFPHSGYERPSNNAIRRFQDLLHQAGYNVTVRTTRGEDIDAACGQLVGQVMDRTRRSE
RYIAVRELSAEADAAPVAVTRT