Protein Info for Psyr_1231 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: protein translocase subunit secF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 130 to 147 (18 residues), see Phobius details amino acids 154 to 176 (23 residues), see Phobius details amino acids 182 to 203 (22 residues), see Phobius details amino acids 229 to 250 (22 residues), see Phobius details amino acids 257 to 283 (27 residues), see Phobius details PF07549: Sec_GG" amino acids 27 to 54 (28 residues), 42.9 bits, see alignment (E = 2.4e-15) TIGR00966: protein-export membrane protein SecF" amino acids 33 to 276 (244 residues), 268.7 bits, see alignment E=5.7e-84 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 90 to 273 (184 residues), 190.6 bits, see alignment E=3.3e-60 PF02355: SecD_SecF" amino acids 109 to 284 (176 residues), 223.1 bits, see alignment E=2.1e-70

Best Hits

Swiss-Prot: 77% identical to SECF_PSEAE: Protein translocase subunit SecF (secF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 100% identity to pst:PSPTO_1416)

MetaCyc: 46% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZX40 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Psyr_1231 protein translocase subunit secF (Pseudomonas syringae pv. syringae B728a ΔmexB)
MLRTINFMGVRNVAFAITMLLTALALFSWFHKGLNFGLDFTGGTLIELTYERPADLGKVR
QELVSAGYHEAVVQSFGATTDLLVRMPGEDPQLGTQVAEALRKAGADNPAVVKRVEFVGP
QVGEELRDQGGLGMLMALGGVLIYLAFRFQWKFAVGAIISLIHDVVVTMGILSFFQITFD
LTVLAAVLAIIGYSLNDTIVVFDRVRENFRLLRKASLIENINISTTQTLLRTIATSVSTL
LAIVALWAFGGDSLEGFSIALFIGVLAGTYSSIYIANVVLIWLNLTTEDLIPPVVVEKAD
DLP