Protein Info for Psyr_1109 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: 6-phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 TIGR01196: phosphogluconate dehydratase" amino acids 2 to 601 (600 residues), 1041.5 bits, see alignment E=0 PF00920: ILVD_EDD" amino acids 67 to 597 (531 residues), 541.2 bits, see alignment E=1.3e-166

Best Hits

Swiss-Prot: 77% identical to EDD_PSEAE: Phosphogluconate dehydratase (edd) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to psb:Psyr_1109)

MetaCyc: 64% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXF9 at UniProt or InterPro

Protein Sequence (608 amino acids)

>Psyr_1109 6-phosphogluconate dehydratase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MHPRVLEVTERLIARSRDTRQRYLQLIRGAASDGPMRGKLQCANFAHGVAACGPEDKQSL
RLMNAANVAIVSSYNEMLSAHQPYEHFPAQIKQALRDIGSVGQFAGGVPAMCDGVTQGEP
GMELAIASREVIAMSTAIALSHNMFDAAMMLGICDKIVPGLMMGALRFGHLPTIFVPGGP
MVSGLSNKEKADVRQRYAEGKATREELLDSEMKSYHGPGTCTFYGTANTNQLVMEVMGMH
LPGASFVNPYTPLRDALTAEAARQVTRMTKQSGSFMPIGEIVDERSLVNSIVALHATGGS
TNHTLHMPAIAQAAGIQLTWQDMADLSEVVPTLSHVYPNGKADINHFQAAGGMSFLIREL
LAAGLLHENVNTVAGYGLSRYTKEPFLEDGKLVWREGPLESLDENILRPVARPFSPEGGL
RVMEGNLGRGVMKVSAVAPEHQIVEAPAKVFQDQKELADAFKAGELECDFVAVMRFQGPR
CNGMPELHKMTPFLGVLQDRGFKVALVTDGRMSGASGKIPAAIHVCPEAFDGGPLALVRD
GDVIRVDGVKGTLQVLVEASELAAREPAINQIDNSVGCGRELFGFMRMAFSSAEQGASAF
TSSLETLK