Protein Info for Psyr_0943 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: LSU ribosomal protein L25P

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 204 TIGR00731: ribosomal protein bL25, Ctc-form" amino acids 6 to 172 (167 residues), 148.7 bits, see alignment E=7.1e-48 PF01386: Ribosomal_L25p" amino acids 6 to 94 (89 residues), 89.7 bits, see alignment E=1.4e-29 PF14693: Ribosomal_TL5_C" amino acids 102 to 188 (87 residues), 90.4 bits, see alignment E=7.6e-30

Best Hits

Swiss-Prot: 100% identical to RL25_PSEU2: 50S ribosomal protein L25 (rplY) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K02897, large subunit ribosomal protein L25 (inferred from 100% identity to psb:Psyr_0943)

Predicted SEED Role

"LSU ribosomal protein L25p" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZXX3 at UniProt or InterPro

Protein Sequence (204 amino acids)

>Psyr_0943 LSU ribosomal protein L25P (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNEFTLNAEQRSDLGKGASRRLRRLASLVPAVVYGGDKAPESISMLAKEVAKLLENDAAY
SHIIELNVGGKKQNVIIKALQRHPAKGHVMHADFVRVIAGQKLTAIVPIHFLNEEAPVKK
GGEISHTTTELEVTCLPKDLPEFIEVDLGSLEVGDNVHLSELKAPKGVEFVALAHGTDLA
IANVHAPRIVKDEEEGEAEEGAAE