Protein Info for Psyr_0798 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: type II secretion system protein E:General secretory system II, protein E, N-terminal

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 431 to 449 (19 residues), see Phobius details TIGR02538: type IV-A pilus assembly ATPase PilB" amino acids 8 to 564 (557 residues), 831 bits, see alignment E=2.6e-254 PF05157: MshEN" amino acids 61 to 147 (87 residues), 55.8 bits, see alignment E=3.8e-19 PF00437: T2SSE" amino acids 177 to 560 (384 residues), 520.6 bits, see alignment E=1.8e-160

Best Hits

Swiss-Prot: 78% identical to PILB_PSEAE: Type 4 fimbrial assembly protein PilB (pilB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02652, type IV pilus assembly protein PilB (inferred from 100% identity to psb:Psyr_0798)

Predicted SEED Role

"Type IV fimbrial assembly, ATPase PilB" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYB6 at UniProt or InterPro

Protein Sequence (564 amino acids)

>Psyr_0798 type II secretion system protein E:General secretory system II, protein E, N-terminal (Pseudomonas syringae pv. syringae B728a ΔmexB)
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQ
FGVPFMDLASLDKESLPKGLVSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTEIQFS
TGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLDIEPADGKETTLATQNDADDA
PVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALG
YEPDQKALYLEALKLPQGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLE
GINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLETAEIAIKASQTGHMVLSTLHT
NSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEAK
IGTFKVYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDL
RTSGLSKAMQGITSLEEVNRVTKD