Protein Info for Psyr_0727 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Protein of unknown function DUF152

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02578: Cu-oxidase_4
  • amino acids 20 to 238 (219 residues), 249.4 bits, E=1.7e-78
• TIGR00726: YfiH family protein
  • amino acids 25 to 239 (215 residues), 202.7 bits, E=2.4e-64

Best Hits

Swiss-Prot: 70% identical to POLOX_PSEAE: Polyphenol oxidase (PA4543) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05810, conserved hypothetical protein (inferred from 100% identity to psb:Psyr_0727)

MetaCyc: 53% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]

Predicted SEED Role

"COG1496: Uncharacterized conserved protein"

MetaCyc Pathways

• L-methionine salvage cycle III (10/11 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• ureide biosynthesis (5/7 steps found)
• 5'-deoxyadenosine degradation I (2/3 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• guanine and guanosine salvage I (1/2 steps found)
• xanthine and xanthosine salvage (1/2 steps found)
• purine deoxyribonucleosides degradation II (1/3 steps found)
• superpathway of guanine and guanosine salvage (1/3 steps found)
• purine ribonucleosides degradation (3/6 steps found)
• purine deoxyribonucleosides degradation I (1/4 steps found)
• superpathway of purine deoxyribonucleosides degradation (2/7 steps found)
• sesamin biosynthesis (1/8 steps found)
• arsenic detoxification (mammals) (7/17 steps found)
• justicidin B biosynthesis (1/10 steps found)
• matairesinol biosynthesis (1/10 steps found)
• nucleoside and nucleotide degradation (archaea) (1/10 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.28, 3.5.4.4

Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4

Sequence Analysis Tools

See Q4ZYH7 at UniProt or InterPro

Protein Sequence (242 amino acids)

>Psyr_0727 Protein of unknown function DUF152 (Pseudomonas syringae pv. syringae B728a ΔmexB)
MNDWLIPDWPAPAQIKSCVTTRSGGVSLAPFDSFNLGDHVDDSPQAVASNRQRLSSSLKA
QPVWLKQVHGIDVVSADPSRVVEADASWTSTPGVACTIMTADCLPALFCRRDGTRVAAAH
AGWRGLAAGVLEATADSLQSAAEDIMVWLGPAIGQPSFEVGPEVREAFTASHPQAAAAFV
ASSNPDRFMADIYALARLRLAAQGISAVYGGGLDTFTDPRFFSYRRAARTGRFASLIWIE
HA