Protein Info for Psyr_0703 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: GTP-binding protein, HSR1-related:GTP1/OBG subdomain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 TIGR02729: Obg family GTPase CgtA" amino acids 3 to 332 (330 residues), 481.2 bits, see alignment E=7.3e-149 PF01018: GTP1_OBG" amino acids 4 to 158 (155 residues), 211.7 bits, see alignment E=6.6e-67 PF01926: MMR_HSR1" amino acids 161 to 284 (124 residues), 80.1 bits, see alignment E=2e-26 PF02421: FeoB_N" amino acids 162 to 217 (56 residues), 27.5 bits, see alignment 3.1e-10

Best Hits

Swiss-Prot: 100% identical to OBG_PSE14: GTPase Obg (obg) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K03979, GTP-binding protein (inferred from 100% identity to psp:PSPPH_0715)

MetaCyc: 62% identical to GTPase ObgE (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Obg"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYK1 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Psyr_0703 GTP-binding protein, HSR1-related:GTP1/OBG subdomain protein (Pseudomonas syringae pv. syringae B728a ΔmexB)
MKFVDEVSIRVKAGDGGNGCMSFRREKFIENGGPNGGDGGDGGSIFMVADVNLNTLVDYR
YTRHFDAERGSNGGSADCTGRKGEELVLRVPVGTTIIDATTQEIIGDLTKDGQRLMVAQG
GWHGLGNTRFKSSTNRAPRQTTPGKPGDQRDLKLELKVLADVGLLGLPNAGKSTFIRSVS
AAKPKVADYPFTTLVPNLGVVSVDRWKSFVVADIPGLIEGASDGAGLGIRFLKHLARTRL
LLHLVDMAPLDESSAPDAAEVIVNELEKFSPSLAERDRWLVLNKCDQILEEEQEARKQEI
VDRLEWTGPVYVISAIAKEGTEQLTRDIMRYLEERSQRIAEEPGYAEELAELDQRIEDEA
RAQLQALDDQRALRRSGVKSVHDIGDDDWDEEDVDDEDGPEIIYVRD