Protein Info for Psyr_0654 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Copper-translocating P-type ATPase:Heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 transmembrane" amino acids 91 to 112 (22 residues), see Phobius details amino acids 120 to 142 (23 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 182 to 201 (20 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details amino acids 363 to 387 (25 residues), see Phobius details amino acids 678 to 696 (19 residues), see Phobius details amino acids 702 to 720 (19 residues), see Phobius details PF00403: HMA" amino acids 10 to 67 (58 residues), 60.5 bits, see alignment 2.5e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 135 to 721 (587 residues), 602.9 bits, see alignment E=1.4e-184 TIGR01525: heavy metal translocating P-type ATPase" amino acids 153 to 720 (568 residues), 624.3 bits, see alignment E=4.9e-191 TIGR01494: HAD ATPase, P-type, family IC" amino acids 191 to 438 (248 residues), 143.9 bits, see alignment E=1.1e-45 amino acids 539 to 701 (163 residues), 139.9 bits, see alignment E=1.8e-44 PF00122: E1-E2_ATPase" amino acids 219 to 397 (179 residues), 185.4 bits, see alignment E=1.2e-58 PF00702: Hydrolase" amino acids 414 to 632 (219 residues), 115.6 bits, see alignment E=5.9e-37

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to psb:Psyr_0654)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZYP8 at UniProt or InterPro

Protein Sequence (732 amino acids)

>Psyr_0654 Copper-translocating P-type ATPase:Heavy metal translocating P-type ATPase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MQDTTTFDLPISGMTCASCAGRVERALAKVPGVNSVTVNLANERAHVDTAAHTDPQTLID
AVSRAGYSATLNQDRHAEADQKARHLHHERWALLLAIVLALPLILPMLLTPLGVHWMLPA
WVQFALATPVQFILGARFYVAAWKAVRAGAGNMDLLVAIGTSAGYGLSVYQWLYAAPGTT
PHLYFEASAVVIALVLLGKYLESRAKRQTASAIRALEALRPERALRVVDGLEQDVAISAL
QLDDRVLVKPGERFPVDGEVVEGRSHADEALITGESLPVVKQPGDNVTGGAINGEGQLLI
RTTALGAETVLARIIRLVEDAQAGKAPIQKLVDKVSQVFVPTVLVIALFTLAGWLLVGAS
LEVALINAVAVLVIACPCSLGLATPTAIMAGTGVAARYGILIKDAEALERAHEVDAVVFD
KTGTLTSGTPRITNMSAVLGNEEHLLQLAGALQRGSEHPLARAVLDVCQEWQLKPDAAQN
SRVLSGRGIAGTVEGRELALGNRRLLEDSGLPMGDLAESARIWEAEGRTLSWLIEQAPEP
KVIGMFAFGDTLKPGTDQAIKALNARGISSHLLTGDNRGSAQVVARALGIHDVHAEVLPA
DKAATVTQLKSLHVVAMVGDGINDAPALAAADVGIAMGGGTDVAMHAAGITLMRGDPRLV
PAALDISRRTYAKIRQNLFWAFVYNLIGIPLAALGYLNPVLAGAAMALSSVSVVSNALLL
KTWKPRDLEDAP