Protein Info for Psyr_0549 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Metallophosphoesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF12850: Metallophos_2" amino acids 13 to 237 (225 residues), 28.6 bits, see alignment E=1.4e-10 PF00149: Metallophos" amino acids 13 to 199 (187 residues), 47.2 bits, see alignment E=3.8e-16

Best Hits

Swiss-Prot: 62% identical to CPDA_PSEAI: 3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA (cpdA) from Pseudomonas aeruginosa

KEGG orthology group: K03651, Icc protein (inferred from 100% identity to psb:Psyr_0549)

MetaCyc: 45% identical to cAMP phosphodiesterase (Escherichia coli K-12 substr. MG1655)
3',5'-cyclic-AMP phosphodiesterase. [EC: 3.1.4.53]

Predicted SEED Role

"3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)" in subsystem cAMP signaling in bacteria (EC 3.1.4.17)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.17

Use Curated BLAST to search for 3.1.4.17 or 3.1.4.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZ03 at UniProt or InterPro

Protein Sequence (268 amino acids)

>Psyr_0549 Metallophosphoesterase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MPSAALSSSVLLVQLSDSHLFAEADGALLGMSTRDSLEKVVDRVLAEQPHIDLVVASGDI
SQDGSVESYEAFRRISGRIDAPARWFAGNHDELPQMEQVAQHNGLLDPVVDIGQWRVTLL
DSAVPGSVPGFLADTQLQLLEQSLSEAPDRHHLICLHHHPVAIGCEWMAPIGLRNADALF
SVLDRFAQVRAILWGHVHQEFDQMRNGVRLLASPSTCIQFAPGSADFKVDTTAPGYRWLR
LHDDGRLETAVSRVVGLEFEVDYGGTGY