Protein Info for Psyr_0500 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: biotin carboxyl carrier protein / biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 109 (109 residues), 146.1 bits, E=1.6e-46
• PF02786: CPSase_L_D2
  • amino acids 114 to 321 (208 residues), 246.8 bits, E=5e-77
• PF02222: ATP-grasp
  • amino acids 137 to 291 (155 residues), 32.5 bits, E=2e-11
• PF02785: Biotin_carb_C
  • amino acids 334 to 443 (110 residues), 132.3 bits, E=2.2e-42
• PF00364: Biotin_lipoyl
  • amino acids 511 to 557 (47 residues), 42.7 bits, 1.2e-14
• PF13533: Biotin_lipoyl_2
  • amino acids 513 to 543 (31 residues), 27 bits, see alignment (E = 9.3e-10)

Best Hits

KEGG orthology group: K11263, acetyl-/propionyl-CoA carboxylase, biotin carboxylase, biotin carboxyl carrier protein [EC: 6.3.4.14] (inferred from 100% identity to psb:Psyr_0500)

MetaCyc: 64% identical to acetyl/propionyl-CoA carboxylase alpha chain (Mycobacterium tuberculosis H37Rv)
RXN0-5055 [EC: 2.1.3.15]

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6) without Allophanate hydrolase 2 domains" (EC 6.3.4.6)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type II) (3/3 steps found)
• urea degradation I (3/3 steps found)
• superpathway of allantoin degradation in yeast (5/6 steps found)
• superpathway of fatty acid biosynthesis initiation (4/5 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• candicidin biosynthesis (1/6 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (9/18 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Fatty acid biosynthesis
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.3.4.6

Use Curated BLAST to search for 2.1.3.15 or 6.3.4.14 or 6.3.4.6

Sequence Analysis Tools

See Q4ZZ52 at UniProt or InterPro

Protein Sequence (577 amino acids)

>Psyr_0500 biotin carboxyl carrier protein / biotin carboxylase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MKKVLIANRGEIAVRIARACRDYGVASVAVYADADIDALHVRQADEAYGLQGERPTDTYL
NIEKLLAVAARAGADAVHPGYGFLSERAEFARAVIDAGLIWIGPDPATIDALGDKVQARR
IALKVGAPLVAGTEDPVNDASEVVAFAEQHGLPIAIKAAFGGGGRGLKVAWKLDEVSELY
ESAVREALTAFGRGECFVERFLDRPRHIEAQIIADRHGRVVVVGTRDCSLQRRNQKLIEE
APAPYLNDELRQRIHESARAVCAEAGYVGAGTVEFLLSGDGTLSFLEVNTRLQVEHPVTE
ETSGVDLVIEQLRVADGLPLSFDGTPVPRGHSFEFRINAEDAGNGFLPTPGSITLFQPPS
GPGVRLDTGVAEGSRVSPNFDSMIAKLIVTGATREQAISRARRALAEFKVEGVATVLPFH
RAVMDHADFTAANTFAVHTRWIETDFAEHVTIAPRNAEPVDPGVLRTFVEIDGKRHELGL
PAELLAGMSLNAAAPADASQPVADAPDPHAVLTPVAGNLHAWAVEDGATVEKGQVIAIME
AMKMETSILAPCAGTVQIGKEPGGYLEAKVVIGRVEA