Protein Info for Psyr_0359 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: D-cysteine desulfhydrase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 61% identical to DCYD_SERP5: D-cysteine desulfhydrase (dcyD) from Serratia proteamaculans (strain 568)

KEGG orthology group: K05396, D-cysteine desulfhydrase [EC: 4.4.1.15] (inferred from 100% identity to psb:Psyr_0359)

MetaCyc: 60% identical to D-cysteine desulfhydrase (Escherichia coli K-12 substr. MG1655)
3-chloro-D-alanine dehydrochlorinase. [EC: 4.5.1.2]; D-cysteine desulfhydrase. [EC: 4.5.1.2, 4.4.1.15]

Predicted SEED Role

"D-cysteine desulfhydrase (EC 4.4.1.15)" (EC 4.4.1.15)

MetaCyc Pathways

superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
glycine betaine degradation III (7/7 steps found)
D-serine degradation (3/3 steps found)
L-serine degradation (3/3 steps found)
L-methionine biosynthesis II (5/6 steps found)
glycine betaine degradation I (6/8 steps found)
L-cysteine degradation II (2/3 steps found)
L-tryptophan degradation II (via pyruvate) (2/3 steps found)
purine nucleobases degradation II (anaerobic) (16/24 steps found)
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
L-mimosine degradation (4/8 steps found)
glutathione-mediated detoxification I (3/8 steps found)

KEGG Metabolic Maps

Cysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.15 or 4.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZJ1 at UniProt or InterPro

Protein Sequence (332 amino acids)

>Psyr_0359 D-cysteine desulfhydrase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MIKHQLARFNRLDLISAPTALEKLERLSIWADRDIYIKRDDTTTLALGGNKVRKLEYLAA
DALAQGADTLITAGAIQSNHVRQTAALAARLGLGCVALLENPIGTEDPSYLKNGNRLLLE
LFDAKVELVENLDNADEQLHALAARLRSSGKKPYLVPIGGSSPVGALGYVRAGLELAEQI
KQTGIEFAAVVLASGSAGTHSGLALALAHELPQLPVIGVTVSRSEEAQLPKVQGLAERTA
ELLGVALPEHFKVELWDEYFAPRYGEPNAGTLSAIKLVASHEGLLLDPVYTGKAMSGLLD
GIGRQRFNDGPLIFLHTGGAPALFAYPDAFDQ