Protein Info for Psyr_0255 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: glutamate-cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 535 transmembrane" amino acids 198 to 217 (20 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 12 to 524 (513 residues), 717.7 bits, see alignment E=3.8e-220 PF04262: Glu_cys_ligase" amino acids 15 to 387 (373 residues), 550.9 bits, see alignment E=5.8e-170

Best Hits

Swiss-Prot: 100% identical to GSH1_PSEU2: Glutamate--cysteine ligase (gshA) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to psb:Psyr_0255)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4ZZU4 at UniProt or InterPro

Protein Sequence (535 amino acids)

>Psyr_0255 glutamate-cysteine ligase (Pseudomonas syringae pv. syringae B728a ΔmexB)
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALT
NGQVTTDYSESLLEFITPALKNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEH
IPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEDAWALLKQTEDFAGDA
RDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNV
LQIENEYYSNIRPKRVTYSGERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDA
FVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLMRDNRPVDLKTWATELMEKIT
PIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN