Protein Info for Psyr_0169 in Pseudomonas syringae pv. syringae B728a ΔmexB

Annotation: Hsp33 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 51 to 64 (14 residues), see Phobius details PF01430: HSP33" amino acids 8 to 279 (272 residues), 247.8 bits, see alignment E=7.3e-78

Best Hits

Swiss-Prot: 100% identical to HSLO_PSEU2: 33 kDa chaperonin (hslO) from Pseudomonas syringae pv. syringae (strain B728a)

KEGG orthology group: K04083, molecular chaperone Hsp33 (inferred from 100% identity to psb:Psyr_0169)

Predicted SEED Role

"33 kDa chaperonin (Heat shock protein 33) (HSP33)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q500D0 at UniProt or InterPro

Protein Sequence (300 amino acids)

>Psyr_0169 Hsp33 protein (Pseudomonas syringae pv. syringae B728a ΔmexB)
MHDFPNIDFTQRFIFDESDVRGELVALERSYAEVLAKHPYPEPVAQLLGELMAAAALLVG
TLKFDGLLILQARSSGAVPLLMVECSSERELRGIARYDEALVTAGAGLQDLMPDGSLALT
IDPNKGKRYQGIVALDGVDLSESLSNYFVMSEQLGTRFWLKADGHRARGLLLQQLPIAQI
TDPEERDASWEHVITLANTLTAEEMLGLDNQTILHRLYHEDPVRLFDEQPICFRCSCSRE
RSANALASLGLEDAQQLVIEHNGTIEIDCQFCNQRYLFDATDVAQLFAGGGVDSPSDTRH